Evidence map›Paper›PMID 26670688›Full record

ArticleJournal of fluorescence2016

Spectroscopy and Molecular Modeling Study on the Interaction Between Mycophenolate Mofetil and Pepsin.

Xiaoli Ma, Liuqi Guo, Qing Wang, Jiawei He, Hui Li

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 28 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xiaoli MaCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
Liuqi GuoCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
Qing WangCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
Jiawei HeCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
Hui LiCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China. lihuilab@sina.com.
Sichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycophenolate mofetil (MMF) is an immunosuppressant used in clinical practice to limit the rejection of transplanted organs. MMF is absorbed and metabolized by the gastrointestinal tract and converted to mycophenolic acid by esterases in the plasma, liver, and kidney. Mycophenolic acid is the resulting active metabolite. The interaction of MMF with pepsin may affect the transfer and distribution of MMF. Given this effect, the present study investigated the interaction behavior between pepsin with MMF using docking simulation and spectroscopy method at different temperatures. The results of spectroscopy revealed that MMF has strong ability to quench the fluorescence of pepsin. The results also show that the acting force for binding was composed of hydrophobic forces. The three-dimensional fluorescence spectra and synchronous spectroscopy employed to determine the conformation showed that the binding of MMF with pepsin could induce MMF conformation and microenvironment changes. Furthermore, the molecular interaction distance and energy-transfer efficiency between pepsin and MMF were determined based on the Förster non-radiative energy-transfer mechanism. Docking simulation showed that MMF entered the hydrophobic cavity of pepsin, and a hydrogen bond was formed between the oxygen atoms of the carbanyl group of MMF and hydrogen atoms of tyrosine 189 of pepsin.

Indexed as

Models, MolecularBinding SitesEnergy TransferFluorescenceHumansHydrophobic and Hydrophilic InteractionsMolecular Docking SimulationMolecular StructureMycophenolic AcidPepsin AProtein BindingProtein ConformationSpectrophotometry, UltravioletMycophenolic AcidPepsin AFluorescence spectroscopyInteractionMolecular modelingMycophenolate mofetilPepsin

Identifiers

PMID26670688
OpenAlexW2275942596

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.