ArticleeLife2015
A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 94 citations in OpenAlex.
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- The Nereid on the rise: Platynereis as a model system.EvoDevo · 2021Review
- Review of Post-embedding Immunogold Methods for the Study of Neuronal Structures.Frontiers in neuroanatomy · 2021Review
- The ventral epithelium ofBiology open · 2019Article
- The appliances and prospects of aurum nanomaterials in biodiagnostics, imaging, drug delivery and combination therapy.Asian journal of pharmaceutical sciences · 2019Review
- Differential Impacts of the Head onFrontiers in physiology · 2019Article
- Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance.eLife · 2018Article
- Whole-head recording of chemosensory activity in the marine annelidOpen biology · 2018Article
- Distinct Co-Modulation Rules of Synapses and Voltage-Gated Currents Coordinate Interactions of Multiple Neuromodulators.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- FluoEM, virtual labeling of axons in three-dimensional electron microscopy data for long-range connectomics.eLife · 2018Article
- Ciliary and rhabdomeric photoreceptor-cell circuits form a spectral depth gauge in marine zooplankton.eLife · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron microscopy-based connectomics aims to comprehensively map synaptic connections in neural tissue. However, current approaches are limited in their capacity to directly assign molecular identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-section transmission electron microscopy (ssTEM) to identify multiple peptidergic neurons in a connectome. The high immunogenicity of neuropeptides and their broad distribution along axons, allowed us to identify distinct neurons by immunolabeling small subsets of sections within larger series. We demonstrate the scalability of siGOLD by using 11 neuropeptide antibodies on a full-body larval ssTEM dataset of the annelid Platynereis. We also reconstruct a peptidergic circuitry comprising the sensory nuchal organs, found by siGOLD to express pigment-dispersing factor, a circadian neuropeptide. Our approach enables the direct overlaying of chemical neuromodulatory maps onto synaptic connectomic maps in the study of nervous systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.