ArticleOptics letters2015
Effect of scanning beam size on the lateral resolution of mouse retinal imaging with SLO.
Article in Optics letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Integrated visible-light optical coherence tomography and fluorescence scanning laser ophthalmoscopy to image retinal ganglion cell axons.Biomedical optics express · 2025Article
- Whole retina light-evoked optoretinography (ORG) under different retina hydration levels: modeling of Bruch's membrane ORGs.Biomedical optics express · 2025Article
- On-axis full-field swept-source optical coherence tomography for murine retinal imaging.Optics letters · 2024Article
- Evaluating the performance of OCT in assessing static and potential dynamic properties of the retinal ganglion cells and nerve fiber bundles in the living mouse eye.Biomedical optics express · 2023Article
- A comparison of optophysiological biomarkers of photoreceptor stress and phototoxicity in BALB/cJ, B6 (Cg)-Tyrc-2J/J, and C57Bl/6J mouse strains.Frontiers in ophthalmology · 2023Article
- Adaptive optics scanning laser ophthalmoscopy and optical coherence tomography (AO-SLO-OCT) system forBiomedical optics express · 2023Article
- Volumetric data analysis enabled spatially resolved optoretinogram to measure the functional signals in the living retina.Journal of biophotonics · 2022Article
- Extraction of phase-based optoretinograms (ORG) from serial B-scans acquired over tens of seconds by mouse retinal raster scanning OCT system.Biomedical optics express · 2021Article
- Retinal analysis of a mouse model of Alzheimer's disease with multicontrast optical coherence tomography.Neurophotonics · 2020Article
- Temporal speckle-averaging of optical coherence tomography volumes forNeurophotonics · 2019Article
- In vivo imaging reveals transient microglia recruitment and functional recovery of photoreceptor signaling after injury.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression.International journal of molecular sciences · 2019Article
- Aperture phase modulation with adaptive optics: a novel approach for speckle reduction and structure extraction in optical coherence tomography.Biomedical optics express · 2019Article
- Effect of a contact lens on mouse retinal in vivo imaging: Effective focal length changes and monochromatic aberrations.Experimental eye research · 2018Article
- In vivo optophysiology reveals that G-protein activation triggers osmotic swelling and increased light scattering of rod photoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Optical modelling of a supplementary tunable air-spaced goggle lens for rodent eye imaging.PloS one · 2017Article
- Intravitreal Administration of Human Bone Marrow CD34+ Stem Cells in a Murine Model of Retinal Degeneration.Investigative ophthalmology & visual science · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Scanning laser ophthalmoscopy (SLO) employs the eye's optics as a microscope objective for retinal imaging in vivo. The mouse retina has become an increasingly important object for investigation of ocular disease and physiology with optogenetic probes. SLO imaging of the mouse eye, in principle, can achieve submicron lateral resolution thanks to a numerical aperture (NA) of ∼0.5, about 2.5 times larger than that of the human eye. In the absence of adaptive optics, however, natural ocular aberrations limit the available optical resolution. The use of a contact lens, in principle, can correct many aberrations, permitting the use of a wider scanning beam and, thus, achieving greater resolution then would otherwise be possible. In this Letter, using an SLO equipped with a rigid contact lens, we report the effect of scanning beam size on the lateral resolution of mouse retinal imaging. Theory predicts that the maximum beam size full width at half-maximum (FWHM) that can be used without any deteriorating effects of aberrations is ∼0.6 mm. However, increasing the beam size up to the diameter of the dilated pupil is predicted to improve lateral resolution, though not to the diffraction limit. To test these predictions, the dendrites of a retinal ganglion cell expressing YFP were imaged, and transverse scans were analyzed to quantify the SLO system resolution. The results confirmed that lateral resolution increases with the beam size as predicted. With a 1.3 mm scanning beam and no high-order aberration correction, the lateral resolution is ∼1.15 μm, superior to that achievable by most human AO-SLO systems. Advantages of this approach include stabilization of the mouse eye and simplified optical design.
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