ArticleProceedings of the National Academy of Sciences of the United States of America2015
Casein kinase II promotes target silencing by miRISC through direct phosphorylation of the DEAD-box RNA helicase CGH-1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- The kinase GSK-3 alters the RNA-binding protein landscape of lipid metabolism transcripts leading to altered expression in the C. elegans nervous system.Nucleic acids research · 2025Article
- Defining the contribution of microRNA-specific Argonautes with slicer capability in animals.Nucleic acids research · 2024Article
- An RNAi screen for conserved kinases that enhance microRNA activity after dauer in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2024Article
- Recent advances in understanding microRNA function and regulation in C. elegans.Seminars in cell & developmental biology · 2024Review
- Site-specific phosphorylation of ZYG-1 regulates ZYG-1 stability and centrosome number.iScience · 2023Article
- Casein kinase 1 and 2 phosphorylate Argonaute proteins to regulate miRNA-mediated gene silencing.EMBO reports · 2023Article
- PLK1 Regulates MicroRNA Biogenesis through Drosha Phosphorylation.International journal of molecular sciences · 2023Article
- Article
- TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.Developmental biology · 2022Article
- Specialized germline P-bodies are required to specify germ cell fate in Caenorhabditis elegans embryos.Development (Cambridge, England) · 2022Article
- Insight into the interaction between the RNA helicase CGH-1 and EDC-3 and its implications.Scientific reports · 2021Article
- Review
- The RabGAP TBC-11 controls Argonaute localization for proper microRNA function in C. elegans.PLoS genetics · 2021Article
- The multifaceted roles of microRNAs in differentiation.Current opinion in cell biology · 2020Review
- HRPK-1, a conserved KH-domain protein, modulates microRNA activity during Caenorhabditis elegans development.PLoS genetics · 2019Article
- Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- DDX54 regulates transcriptome dynamics during DNA damage response.Genome research · 2017Article
- Casein kinase II is required for proper cell division and acts as a negative regulator of centrosome duplication in Caenorhabditis elegans embryos.Biology open · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) play essential, conserved roles in diverse developmental processes through association with the miRNA-induced silencing complex (miRISC). Whereas fundamental insights into the mechanistic framework of miRNA biogenesis and target gene silencing have been established, posttranslational modifications that affect miRISC function are less well understood. Here we report that the conserved serine/threonine kinase, casein kinase II (CK2), promotes miRISC function in Caenorhabditis elegans. CK2 inactivation results in developmental defects that phenocopy loss of miRISC cofactors and enhances the loss of miRNA function in diverse cellular contexts. Whereas CK2 is dispensable for miRNA biogenesis and the stability of miRISC cofactors, it is required for efficient miRISC target mRNA binding and silencing. Importantly, we identify the conserved DEAD-box RNA helicase, CGH-1/DDX6, as a key CK2 substrate within miRISC and demonstrate phosphorylation of a conserved N-terminal serine is required for CGH-1 function in the miRNA pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.