ArticleIranian journal of microbiology2015
Genotyping of Haemophilus influenzae type b strains and their incidence in the clinical samples isolated from Iranian patients.
Article in Iranian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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Who cites it
4 citing papers in PubMed.
- Review
- Non-TypeableInternational journal of molecular sciences · 2020Article
- Cloning and sequencing of theIranian journal of microbiology · 2019Article
- MLVA typing ofIranian journal of microbiology · 2018Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveHaemophilus influenzae type b (Hib) is divided into two distinct genotypes, type I and type II, based on the structure of capsular polysaccharides. The capsulation locus of Haemophilus influenzae type b consists of three functionally distinct regions, designated regions 1 to 3. Region III contains hcsA and hcsB genes; however, notable sequence variation in this region can be used to recognize different Hib genotypes. The purpose of this study was to investigate the prevalence and genotype of the Hib strains isolated from patients with invasive disease in Iran. MATERIALS AND
methodsIn the present study, 8 pairs of primers were used for identification and serotyping of encapsulated Haemophilus influenzae strains, as well as confirmation of species identification. Additionally, in order to identify the capsular genotypes of Haemophilus influenzae type b (type I and II), two additional primer pairs were used to amplify the hcsA gene.
resultsOut of 50 isolates of H. influenzae, four were found to be type b. Interestingly, among these 4 Hib isolates, 2 strains belonged to the type-II category.
conclusionOur study shows that the prevalence of both Hib types I and II seems to be high in Iran.
Indexed as
Identifiers
26668700PMC4676982What OpenQuestion holds
Registered trials
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