ArticleThe Journal of cell biology2015
Whole-proteome genetic analysis of dependencies in assembly of a vertebrate kinetochore.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 49 citations in OpenAlex.
- Linking kinetochore attachment to checkpoint control: the role of Aurora B in BubR1 acetylation.Nucleic acids research · 2026Article
- Article
- Sustainable integrative cell biology: CENP-C is guilty by association.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- PBRM1 directs PBAF to pericentromeres and protects centromere integrity.Nature communications · 2025Article
- Genetic Foundation of Male Spur Length and Its Correlation with Female Egg Production in Chickens.Animals : an open access journal from MDPI · 2024Article
- A conserved CENP-E region mediates BubR1-independent recruitment to the outer corona at mitotic onset.Current biology : CB · 2024Article
- Cell cycle control of kinetochore assembly.Nucleus (Austin, Tex.) · 2022Review
- Review
- The intrinsically disorderly story of Ki-67.Open biology · 2021Review
- The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle.Open biology · 2020Review
- A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation.Nucleic acids research · 2020Article
- CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes.The Journal of cell biology · 2020Article
- Heat Shock Cognate 70 Functions as A Chaperone for the Stability of Kinetochore Protein CENP-N in Holocentric Insect Silkworms.International journal of molecular sciences · 2019Article
- In vitro BioID: mapping the CENP-A microenvironment with high temporal and spatial resolution.Molecular biology of the cell · 2019Article
- Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.Molecular cell · 2018Article
- An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.eLife · 2018Article
- Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.Journal of cell science · 2018Article
- Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis.Journal of cell science · 2018Article
- Kinase and Phosphatase Cross-Talk at the Kinetochore.Frontiers in cell and developmental biology · 2018Review
- Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Kinetochores orchestrate mitotic chromosome segregation. Here, we use quantitative mass spectrometry of mitotic chromosomes isolated from a comprehensive set of chicken DT40 mutants to examine the dependencies of 93 confirmed and putative kinetochore proteins for stable association with chromosomes. Clustering and network analysis reveal both known and unexpected aspects of coordinated behavior for members of kinetochore protein complexes. Surprisingly, CENP-T depends on CENP-N for chromosome localization. The Ndc80 complex exhibits robust correlations with all other complexes in a "core" kinetochore network. Ndc80 associated with CENP-T interacts with a cohort of Rod, zw10, and zwilch (RZZ)-interacting proteins that includes Spindly, Mad1, and CENP-E. This complex may coordinate microtubule binding with checkpoint signaling. Ndc80 associated with CENP-C forms the KMN (Knl1, Mis12, Ndc80) network and may be the microtubule-binding "workhorse" of the kinetochore. Our data also suggest that CENP-O and CENP-R may regulate the size of the inner kinetochore without influencing the assembly of the outer kinetochore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.