Evidence map›Paper›PMID 26668208›Full record

ArticleThe Journal of endocrinology2016

Minimal impact of age and housing temperature on the metabolic phenotype of Acc2-/- mice.

Amanda E Brandon, Ella Stuart, Simon J Leslie, Kyle L Hoehn, David E James, Edward W Kraegen, Nigel Turner, Gregory J Cooney

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Amanda E BrandonDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Diabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Ella StuartDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Simon J LeslieDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Kyle L HoehnDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
David E JamesDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Edward W KraegenDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Diabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Nigel TurnerDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Gregory J CooneyDiabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia Diabetes and Metabolism DivisionGarvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, AustraliaSt Vincent's Clinical SchoolUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Medical SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Biotechnology and Biomolecular SciencesUniversity of New South Wales Australia, Sydney, New South Wales, AustraliaSchool of Molecular Bioscience and Sydney Medical SchoolCharles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia g.cooney@garvan.org.au.
UNSW Sydney · AUGarvan Institute of Medical Research · AUUniversity of Sydney · AU

Funding

ACC enzymes and protein acetylationR01DK101803 · NIDDK · UNIVERSITY OF VIRGINIA · PI HOEHN, KYLE LEE · 2014 to 2017
$1.4M
Lipid Metabolism in Ethanol-Stimulated Liver CancerR21AA022154 · NIAAA · UNIVERSITY OF VIRGINIA · PI HOEHN, KYLE LEE · 2014 to 2015
$409k
NIAAA NIH HHS R21 AA022154NIDDK NIH HHS R01 DK101803
6 · The paper itself

Abstract

An important regulator of fatty acid oxidation (FAO) is the allosteric inhibition of CPT-1 by malonyl-CoA produced by the enzyme acetyl-CoA carboxylase 2 (ACC2). Initial studies suggested that deletion of Acc2 (Acacb) increased fat oxidation and reduced adipose tissue mass but in an independently generated strain of Acc2 knockout mice we observed increased whole-body and skeletal muscle FAO and a compensatory increase in muscle glycogen stores without changes in glucose tolerance, energy expenditure or fat mass in young mice (12-16 weeks). The aim of the present study was to determine whether there was any effect of age or housing at thermoneutrality (29 °C; which reduces total energy expenditure) on the phenotype of Acc2 knockout mice. At 42-54 weeks of age, male WT and Acc2(-/-) mice had similar body weight, fat mass, muscle triglyceride content and glucose tolerance. Consistent with younger Acc2(-/-) mice, aged Acc2(-/-) mice showed increased whole-body FAO (24 h average respiratory exchange ratio=0.95±0.02 and 0.92±0.02 for WT and Acc2(-/-) mice respectively, P<0.05) and skeletal muscle glycogen content (+60%, P<0.05) without any detectable change in whole-body energy expenditure. Hyperinsulinaemic-euglycaemic clamp studies revealed no difference in insulin action between groups with similar glucose infusion rates and tissue glucose uptake. Housing Acc2(-/-) mice at 29 °C did not alter body composition, glucose tolerance or the effects of fat feeding compared with WT mice. These results confirm that manipulation of Acc2 may alter FAO in mice, but this has little impact on body composition or insulin action.

Indexed as

Housing, AnimalTemperatureAcetyl-CoA CarboxylaseAgingAnimalsBody CompositionBody WeightEnergy MetabolismFatty AcidsGlucose Clamp TechniqueGlucose Tolerance TestGlycogenInsulinMaleMiceMice, KnockoutAcacb protein, mouseAcetyl-CoA CarboxylaseFatty AcidsGlycogenInsulinTriglyceridesacetyl CoA carboxylaseenergy balanceglucose metabolisminsulin resistancemetabolism

Identifiers

PMID26668208
PMCPMC4906541
OpenAlexW2274588523

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.