Evidence map›Paper›PMID 26667932›Full record

ArticleClinical & experimental metastasis2016

Epithelial mesenchymal-like transition occurs in a subset of cells in castration resistant prostate cancer bone metastases.

Maahum Haider, Xiaotun Zhang, Ilsa Coleman, Nolan Ericson, Lawrence D True, Hung-Ming Lam, Lisha G Brown, Melanie Ketchanji, Belinda Nghiem, Bryce Lakely and 9 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical & experimental metastasis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

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  14. The Movember Global Action Plan 1 (GAP1): Unique Prostate Cancer Tissue Microarray Resource.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 1 country.

Maahum HaiderGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Xiaotun ZhangGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Ilsa ColemanDivison of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Nolan EricsonDivison of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Lawrence D TrueDepartment of Pathology, University of Washington, Seattle, WA, USA.
Hung-Ming LamGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Lisha G BrownGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Melanie KetchanjiGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Belinda NghiemGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Bryce LakelyGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Roger ColemanDivison of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Bruce MontgomeryDepartment of Medicine, University of Washington, Seattle, WA, USA.
Paul H LangeGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Martine RoudierGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Celestia S HiganoGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Jason H BielasDivison of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Peter S NelsonDivison of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Robert L VessellaGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA.
Colm MorrisseyGenitourinary Cancer Research Laboratory, Department of Urology, University of Washington, Box 356510, Seattle, WA, 98195, USA. cmorriss@u.washington.edu.
University of Washington · USFred Hutch Cancer Center · US

Funding

TRANSCRIPTOME AND PROTEOME STRATIFICATION OF PROSTATE ADENOCARCINOMA PHENOTYPESP50CA097186 · NCI · FRED HUTCHINSON CANCER RESEARCH CENTER · PI John Kyung Lee · 2002 to 2026
$58.1M
Transmembrane Proteases and Prostate CarcinogenesisP01CA085859 · NCI · UNIVERSITY OF WASHINGTON · PI VESSELLA, ROBERT LOUIS · 2002 to 2013
$21.2M
Mechanisms of Environmental and Nuclear and Mitochondrial MutagenesisR01ES019319 · NIEHS · FRED HUTCHINSON CANCER RESEARCH CENTER · PI BIELAS, JASON H · 2010 to 2014
$2.5M
NCI NIH HHS P01 CA085859NCI NIH HHS P01CA085859NCI NIH HHS P50 CA097186NCI NIH HHS P50CA97186NIEHS NIH HHS R01 ES019319
6 · The paper itself

Abstract

TGFβ is a known driver of epithelial-mesenchymal transition (EMT) which is associated with tumor aggressiveness and metastasis. However, EMT has not been fully explored in clinical specimens of castration-resistant prostate cancer (CRPC) metastases. To assess EMT in CRPC, gene expression analysis was performed on 149 visceral and bone metastases from 62 CRPC patients and immunohistochemical analysis was performed on 185 CRPC bone and visceral metastases from 42 CRPC patients. In addition, to assess the potential of metastases to seed further metastases the mitochondrial genome was sequenced at different metastatic sites in one patient. TGFβ was increased in bone versus visceral metastases. While primarily cytoplasmic; nuclear and cytoplasmic Twist were significantly higher in bone than in visceral metastases. Slug and Zeb1 were unchanged, with the exception of nuclear Zeb1 being significantly higher in visceral metastases. Importantly, nuclear Twist, Slug, and Zeb1 were only present in a subset of epithelial cells that had an EMT-like phenotype. Underscoring the relevance of EMT-like cells, mitochondrial sequencing revealed that metastases could seed additional metastases in the same patient. In conclusion, while TGFβ expression and EMT-associated protein expression is present in a considerable number of CRPC visceral and bone metastases, nuclear Twist, Slug, and Zeb1 localization and an EMT-like phenotype (elongated nuclei and cytoplasmic compartment) was only present in a small subset of CRPC bone metastases. Mitochondrial sequencing from different metastases in a CRPC patient provided evidence for the seeding of metastases from previously established metastases, highlighting the biological relevance of EMT-like behavior in CRPC metastases.

Indexed as

Bone NeoplasmsEpithelial-Mesenchymal TransitionGene Expression ProfilingHomeodomain ProteinsHumansImmunohistochemistryLaser Capture MicrodissectionMaleNeoplasm MetastasisNuclear ProteinsProstatic Neoplasms, Castration-ResistantReal-Time Polymerase Chain ReactionSnail Family Transcription FactorsTissue Array AnalysisTranscription FactorsTranscriptomeHomeodomain ProteinsNuclear ProteinsSNAI1 protein, humanSnail Family Transcription FactorsTranscription FactorsTransforming Growth Factor betaTWIST1 protein, humanTwist-Related Protein 1ZEB1 protein, humanZinc Finger E-box-Binding Homeobox 1Bone metastasisEpithelial mesenchymal transitionProstate cancerTGFβ

Identifiers

PMID26667932
PMCPMC4777655
OpenAlexW2212554756

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.