ArticleJournal of the American Chemical Society2016
Biosynthesis and Reactivity of Cysteine Persulfides in Signaling.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
116 citing papers in PubMed, 243 citations in OpenAlex.
- What do we know about redox status? A review in neonates of mothers with preeclampsia.Annals of medicine · 2026Review
- Ultrasensitive bioorthogonal probes for selective discrimination of trace HActa pharmaceutica Sinica. B · 2026Article
- Article
- Endogenous Sulfane Sulfur Mediates the Oxidative Stress Response Process inAntioxidants (Basel, Switzerland) · 2026Article
- Cystine C-S bond cleavage fuels cysteine production under disulfide reductase deficiency.Nature chemical biology · 2026Article
- Multifaceted roles for persulfide species in redox chemical biology.Nature chemical biology · 2026Review
- Self-bioRxiv : the preprint server for biology · 2026Article
- Distinct control of T cell proliferation and effector function by partitioning of intracellular sulfur from cysteine.bioRxiv : the preprint server for biology · 2026Article
- Physiological Roles of Hydrogen Sulfide/Polysulfides and Regulation of their Production.Handbook of experimental pharmacology · 2026Review
- Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and HNature communications · 2025Article
- Digitonin-Loaded Nanoscale Metal-Organic Framework for Mitochondria-Targeted Radiotherapy-Radiodynamic Therapy and Disulfidptosis.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- The potential role of hydrogen sulfide-autophagy axis in diseases.Journal of physiology and biochemistry · 2025Review
- Profiling the landscape of cysteine posttranslational modifications in brain aging and neurodegeneration.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Review
- Electrospun Polymer Fiber Mats for Persulfide Prodrug Delivery.Biomacromolecules · 2025Article
- The Biological Consequences of the Knockout of Genes Involved in the Synthesis and Metabolism of HAntioxidants (Basel, Switzerland) · 2025Article
- Knockout of the sulfide: quinone oxidoreductase SQR reduces growth of HCT116 tumor xenograft.Redox biology · 2025Article
- Serendipitous Discovery of Photolytic Thiosulfoxide Formation: Application for Visible-Light-Inducible Manipulation of Supersulfide Level in Biological Systems.Journal of the American Chemical Society · 2025Article
- The Reliable Detection of Homocysteine Using a Biosensor Based on Recombinant Cystathionine β-Synthase and Nanoporous Gold.Microorganisms · 2025Article
- Supersulfide controls intestinal inflammation by suppressing CD4Frontiers in immunology · 2025Article
- Quantification of persulfidation on specific proteins: are we nearly there yet?Essays in biochemistry · 2024Review
56 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Hydrogen sulfide (H2S) elicits pleiotropic physiological effects ranging from modulation of cardiovascular to CNS functions. A dominant method for transmission of sulfide-based signals is via posttranslational modification of reactive cysteine thiols to persulfides. However, the source of the persulfide donor and whether its relationship to H2S is as a product or precursor is controversial. The transsulfuration pathway enzymes can synthesize cysteine persulfide (Cys-SSH) from cystine and H2S from cysteine and/or homocysteine. Recently, Cys-SSH was proposed as the primary product of the transsulfuration pathway with H2S representing a decomposition product of Cys-SSH. Our detailed kinetic analyses demonstrate a robust capacity for Cys-SSH production by the human transsulfuration pathway enzymes, cystathionine beta-synthase and γ-cystathionase (CSE) and for homocysteine persulfide synthesis from homocystine by CSE only. However, in the reducing cytoplasmic milieu where the concentration of reduced thiols is significantly higher than of disulfides, substrate level regulation favors the synthesis of H2S over persulfides. Mathematical modeling at physiologically relevant hepatic substrate concentrations predicts that H2S rather than Cys-SSH is the primary product of the transsulfuration enzymes with CSE being the dominant producer. The half-life of the metastable Cys-SSH product is short and decomposition leads to a mixture of polysulfides (Cys-S-(S)n-S-Cys). These in vitro data, together with the intrinsic reactivity of Cys-SSH for cysteinyl versus sulfur transfer, are consistent with the absence of an observable increase in protein persulfidation in cells in response to exogenous cystine and evidence for the formation of polysulfides under these conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.