Evidence map›Paper›PMID 26667178›Full record

ArticleJournal of the American Society for Mass Spectrometry2016

Ambient Molecular Analysis of Biological Tissue Using Low-Energy, Femtosecond Laser Vaporization and Nanospray Postionization Mass Spectrometry.

Fengjian Shi, Paul M Flanigan, Jieutonne J Archer, Robert J Levis

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020
    Review
  2. Quantification of Protein-Ligand Interactions by Laser Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Fengjian ShiDepartment of Chemistry, Temple University, 1901 N. 13th St., Philadelphia, PA, 19122, USA.
Paul M FlaniganDepartment of Chemistry, Temple University, 1901 N. 13th St., Philadelphia, PA, 19122, USA.
Jieutonne J ArcherDepartment of Chemistry, Temple University, 1901 N. 13th St., Philadelphia, PA, 19122, USA.
Robert J LevisDepartment of Chemistry, Temple University, 1901 N. 13th St., Philadelphia, PA, 19122, USA. rjlevis@temple.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Direct analysis of plant and animal tissue samples by laser electrospray mass spectrometry (LEMS) was investigated using low-energy, femtosecond duration laser vaporization at wavelengths of 800 and 1042 nm followed by nanospray postionization. Low-energy (<50 μJ), fiber-based 1042 nm LEMS (F-LEMS) allowed interrogation of the molecular species in fresh flower petal and leaf samples using 435 fs, 10 Hz bursts of 20 pulses from a Ytterbium-doped fiber laser and revealed comparable results to high energy (75-1120 μJ), 45 fs, 800 nm Ti:Sapphire-based LEMS (Ti:Sapphire-LEMS) measurements. Anthocyanins, sugars, and other metabolites were successfully detected and revealed the anticipated metabolite profile for the petal and leaf samples. Phospholipids, especially phosphatidylcholine, were identified from a fresh mouse brain section sample using Ti:Sapphire-LEMS without the application of matrix. These lipid features were suppressed in both the fiber-based and Ti:Sapphire-based LEMS measurements when the brain sample was prepared using the optimal cutting temperature compounds that are commonly used in animal tissue cryosections.

Indexed as

Brain ChemistryAnimalsAnthocyaninsCarbohydratesEquipment DesignLasersMicePhospholipidsPlantsSpectrometry, Mass, Electrospray IonizationVolatilizationAnthocyaninsCarbohydratesPhospholipidsAmbient mass spectrometryBiological tissueElectrospray ionizationFemtosecond fiber laserLaser vaporization

Identifiers

PMID26667178

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.