Evidence map›Paper›PMID 26666990›Full record

ArticleMicrobiologyOpen2016

Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach.

Birgit Uytterhoeven, Kenny Appermans, Lijiang Song, Joleen Masschelein, Thomas Lathouwers, Chris W Michiels, Rob Lavigne

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Birgit UytterhoevenLaboratory of Gene Technology, KU Leuven, Kasteelpark Arenberg 21 box 2462, Heverlee, B-3001, Belgium.
Kenny AppermansLaboratory of Gene Technology, KU Leuven, Kasteelpark Arenberg 21 box 2462, Heverlee, B-3001, Belgium.
Lijiang SongDepartment of Chemistry, University of Warwick, Gibbet Hill, Coventry, CV4 7AL, United Kingdom.
Joleen MasscheleinLaboratory of Gene Technology, KU Leuven, Kasteelpark Arenberg 21 box 2462, Heverlee, B-3001, Belgium.
Thomas LathouwersLaboratory of Gene Technology, KU Leuven, Kasteelpark Arenberg 21 box 2462, Heverlee, B-3001, Belgium.
Chris W MichielsCentre for Food and Microbial Technology, KU Leuven, Kasteelpark Arenberg 23 box 2457, Heverlee, B-3001, Belgium.
Rob LavigneLaboratory of Gene Technology, KU Leuven, Kasteelpark Arenberg 21 box 2462, Heverlee, B-3001, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans-acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359. We here present a systematic analysis of the substrate specificity of the glycine-incorporating adenylation domain from the kalimantacin biosynthetic assembly line by a targeted mutagenesis approach. The specificity-conferring code was adapted for use in Pseudomonas and mutated adenylation domain active site sequences were introduced in the kalimantacin gene cluster, using a newly adapted ligation independent cloning method. Antimicrobial activity screens and LC-MS analyses revealed that the production of the kalimantacin analogues in the mutated strains was abolished. These results support the idea that further insight in the specificity of downstream domains in nonribosomal peptide synthetases and polyketide synthases is required to efficiently engineer these strains in vivo.

Indexed as

MutagenesisBiosynthetic PathwaysCarbamatesCatalytic DomainChromatography, LiquidCloning, MolecularCodonGenes, BacterialMass SpectrometryMutationPeptide SynthasesProtein Interaction Domains and MotifsPseudomonas fluorescensSubstrate SpecificityCarbamatesCodonnon-ribosomal peptide synthasePeptide SynthasesAdenylation domainkalimantacinligation independent cloningspecificity-conferring code

Identifiers

PMID26666990
PMCPMC4831472

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.