ArticleNanoscale research letters2015
Discrimination Between Cervical Cancer Cells and Normal Cervical Cells Based on Longitudinal Elasticity Using Atomic Force Microscopy.
Article in Nanoscale research letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- The higher cellular elastic modulus of the nucleus pulposus with degenerative changes in the cervical intervertebral discs.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2026Article
- Probing Nanomechanics by Direct Indentation Using Nanoendoscopy-AFM Reveals the Nuclear Elasticity Transition in Cancer Cells.ACS applied nano materials · 2025Article
- Review
- Insights into the mechanobiology of cancer metastasis via microfluidic technologies.APL bioengineering · 2024Review
- Mechanical properties of human tumour tissues and their implications for cancer development.Nature reviews. Physics · 2024Article
- Classification of cancer cells at the sub-cellular level by phonon microscopy using deep learning.Scientific reports · 2023Article
- Review
- Indenting soft samples (hydrogels and cells) with cantilevers possessing various shapes of probing tip.European biophysics journal : EBJ · 2020Article
- Application of atomic force microscope in diagnosis of single cancer cells.Biomicrofluidics · 2020Review
- Cortical stiffness of keratinocytes measured by lateral indentation with optical tweezers.PloS one · 2020Article
- The High Mobility Group A1 (HMGA1) Chromatin Architectural Factor Modulates Nuclear Stiffness in Breast Cancer Cells.International journal of molecular sciences · 2019Article
- Regional biomechanical imaging of liver cancer cells.Journal of Cancer · 2019Article
- Application of atomic force microscopy in cancer research.Journal of nanobiotechnology · 2018Review
- Design of a Novel MEMS Microgripper with Rotatory Electrostatic Comb-Drive Actuators for Biomedical Applications.Sensors (Basel, Switzerland) · 2018Article
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
- Nanomechanical Characteristics of Cervical Cancer and Cervical Intraepithelial Neoplasia Revealed by Atomic Force Microscopy.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Biophysical Approach to Mechanisms of Cancer Prevention and Treatment with Green Tea Catechins.Molecules (Basel, Switzerland) · 2016Review
- Characterizing the malignancy and drug resistance of cancer cells from their membrane resealing response.Scientific reports · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical properties of cells are considered promising biomarkers for the early diagnosis of cancer. Recently, atomic force microscopy (AFM)-based nanoindentation technology has been utilized for the examination of cell cortex mechanics in order to distinguish malignant cells from normal cells. However, few attempts to evaluate the biomechanical properties of cells have focused on the quantification of the non-homogeneous longitudinal elasticity of cellular structures. In the present study, we applied a variation of the method of Carl and Schillers to investigate the differences between longitudinal elasticity of human cervical squamous carcinoma cells (CaSki) and normal cervical epithelial cells (CRL2614) using AFM. The results reveal a three-layer heterogeneous structure in the probing volume of both cell types studied. CaSki cells exhibited a lower whole-cell stiffness and a softer nuclei zone compared to the normal counterpart cells. Moreover, a better differentiated cytoskeleton was found in the inner cytoplasm/nuclei zone of the normal CRL2614 cells, whereas a deeper cytoskeletal distribution was observed in the probing volume of the cancerous counterparts. The sensitive cortical panel of CaSki cells, with a modulus of 0.35~0.47 kPa, was located at 237~225 nm; in normal cells, the elasticity was 1.20~1.32 kPa at 113~128 nm. The present improved method may be validated using the conventional Hertz-Sneddon method, which is widely reported in the literature. In conclusion, our results enable the quantification of the heterogeneous longitudinal elasticity of cancer cells, in particular the correlation with the corresponding depth. Preliminary results indicate that our method may potentially be applied to improve the detection of cancerous cells and provide insights into the pathophysiology of the disease.
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