ArticleScience advances2015
Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth.
Article in Science advances, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Revisiting fungal polarized growth: from Spitzenkörper to crescent accumulation of secretory vesicles.iScience · 2025Article
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling.Communications biology · 2024Article
- Hyphae of the fungus Aspergillus nidulans demonstrate chemotropism to nutrients and pH.PLoS biology · 2024Article
- Structural diversification of fungal cell wall in response to the stress signaling and remodeling during fungal pathogenesis.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Kinesin-4 optimizes microtubule orientations for responsive tip growth guidance in moss.The Journal of cell biology · 2023Article
- Chitin Biosynthesis inJournal of fungi (Basel, Switzerland) · 2023Review
- Raman Micro-spectroscopy and Imaging of Filamentous Fungi.Microbes and environments · 2022Review
- Turning Inside Out: Filamentous Fungal Secretion and Its Applications in Biotechnology, Agriculture, and the Clinic.Journal of fungi (Basel, Switzerland) · 2021Review
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- Application of PALM Superresolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Dynamics of the HIV Gag Lattice Detected by Localization Correlation Analysis and Time-Lapse iPALM.Biophysical journal · 2020Article
- Single-Molecule Localization Microscopy to Study Protein Organization in the Filamentous FungusMolecules (Basel, Switzerland) · 2020Article
- Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories.Biotechnology for biofuels · 2019Review
- Functional exploration of co-expression networks identifies a nexus for modulating protein and citric acid titres inFungal biology and biotechnology · 2019Article
- Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion.Microbial cell factories · 2018Article
- Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures.Microbiology and molecular biology reviews : MMBR · 2018Review
- Endocytic recycling via the TGN underlies the polarized hyphal mode of life.PLoS genetics · 2018Article
- Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position inFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 4 countries.
Funding
Abstract
Polar (directional) cell growth, a key cellular mechanism shared among a wide range of species, relies on targeted insertion of new material at specific locations of the plasma membrane. How these cell polarity sites are stably maintained during massive membrane insertion has remained elusive. Conventional live-cell optical microscopy fails to visualize polarity site formation in the crowded cell membrane environment because of its limited resolution. We have used advanced live-cell imaging techniques to directly observe the localization, assembly, and disassembly processes of cell polarity sites with high spatiotemporal resolution in a rapidly growing filamentous fungus, Aspergillus nidulans. We show that the membrane-associated polarity site marker TeaR is transported on microtubules along with secretory vesicles and forms a protein cluster at that point of the apical membrane where the plus end of the microtubule touches. There, a small patch of membrane is added through exocytosis, and the TeaR cluster gets quickly dispersed over the membrane. There is an incessant disassembly and reassembly of polarity sites at the growth zone, and each new polarity site locus is slightly offset from preceding ones. On the basis of our imaging results and computational modeling, we propose a transient polarity model that explains how cell polarity is stably maintained during highly active directional growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.