ArticleFrontiers in veterinary science2014
Characterization of the Vaginal Microbiota of Ewes and Cows Reveals a Unique Microbiota with Low Levels of Lactobacilli and Near-Neutral pH.
Article in Frontiers in veterinary science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed, 175 citations in OpenAlex.
- Characterization of the vaginal bacterial microbiota of sheep exhibiting abortion or repeat breeder syndrome.Veterinary and animal science · 2026Article
- Intravaginal Probiotics in Transition Dairy Cows: A Randomized Multi-Farm Field Trial on Health and Milk Production.Veterinary sciences · 2026Article
- Metagenomic analysis of the vaginal microbiota in cows with ovarian cysts.Journal of veterinary research · 2026Article
- One Health Investigation of Stage-Dependent Antimicrobial Resistance Patterns Across Intermediate and Ripened Dairy Matrices: The Tyrovolia-Kopanisti Paradigm.Microorganisms · 2026Article
- Vaginal Microbiome Is Associated with Breed and Pregnancy Status in Beef Cattle.Animals : an open access journal from MDPI · 2026Article
- Effects of feeding a Bacillus-based probiotic on nutrient utilization and microbiota profile in the rumen and reproductive tract of beef heifers receiving a forage-based diet.Journal of animal science · 2026Article
- Shifts in the vaginal microbiota of nulliparous ewe lambs following initial reproductive handling and estrus synchronization.Journal of animal science · 2026Article
- Semen quality and bacteriological profile of semen collected by three methods and fertility in Huacaya alpacas (Frontiers in veterinary science · 2026Article
- Development of a thermosensitive enrofloxacin hydrogel suspension for intrauterine infusion in cattle.Frontiers in veterinary science · 2026Article
- Impact of whole-herd vaccination on a caprine coxiellosis outbreak: a longitudinal study ofFrontiers in microbiology · 2026Article
- Eicosanoid-immunometabotypes reveal genotypic links to cervicovaginal pH in progestagen synchronised South African Dohne Merino ewes.Frontiers in veterinary science · 2026Article
- Metabarcoding analysis of the microbiota in flocks naturally infected byOne health (Amsterdam, Netherlands) · 2025Article
- Characterization of bacterial communities of ewe's vaginal tract and its potential impact on reproductive efficiency.Animal microbiome · 2025Article
- Identification of Naturally Occurring Inhabitants of Vaginal Microbiota in Cows and Determination of Their Antibiotic Sensitivity.Veterinary sciences · 2025Article
- The cervical microbiome of ewe breeds with known divergent fertility following artificial insemination with frozen-thawed semen.Scientific reports · 2025Article
- Discovery of reproductive tissue-associated bacteria and the modes of microbiota acquisition in male honey bees (drones).mSphere · 2025Article
- Vaginal microbiota and circulating interferon-stimulated genes in lactating dairy cows at and following time of artificial insemination.Journal of animal science · 2025Article
- Evaluating the inoculating bacterial communities within the bovine neonatal respiratory tract after applying an ablation technique to the dam vaginal microbiota prior to parturition.Journal of animal science · 2025Article
- Article
- Fundamentals of microbiome-based therapies for reproductive tract inflammatory diseases in domestic animals.Animal reproduction · 2025Review
40 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although a number of common reproductive disorders in livestock involve bacterial infection, very little is known about their normal vaginal microbiota. Therefore, we sought to determine the species composition of sheep and cattle vaginal microbiota. Twenty Rambouillet ewes and twenty crossbred cows varying in age and reproductive status were sampled by ectocervicovaginal lavage. We amplified and sequenced the V3-V4 region of the 16S ribosomal RNA (rRNA) contents yielding a total of 907,667 high-quality reads. Good's Coverage estimates indicated that we obtained data on 98 ± 0.01% of the total microbial genera present in each sample. Cow and ewe vaginal microbiota displayed few differences. Cow microbiota exhibited greater (P ≤ 0.05) α-diversity compared to the ewe microbiota. Both livestock species differed (P ≤ 0.05) from all previously reported vaginal communities. While bacteria were numerically dominant, Archaea were detected in 95% of cow and ewe samples, mainly of the order Desulfurococcales. Both ewes and cows were predominately colonized by the bacterial phyla Bacteroidetes, Fusobacteria, and Proteobacteria. The most abundant genera were Aggregatibacter spp., and Streptobacillus spp. Lactobacillus spp. were detected in 80% of ewe and 90% of cow samples, but only at very low abundances. Bacteria previously described from culture-based studies as common to the cow and ewe vaginal tract, except for Escherichia, were variably present, and only in low abundance. Ewe and cow pH differed (P ≤ 0.05), with means (±SD) of 6.7 ± 0.38 and 7.3 ± 0.63, respectively. In conclusion, 16S rRNA sequencing of cow and ewe vaginal ectocervicovaginal lavages showed that cow and ewe vaginal microbiota differ from culture-led results, revealing a microbiota distinct from previously described vaginal ecosystems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.