Evidence map›Paper›PMID 26663987›Full record

ArticleMediators of inflammation2015

Altered Innate Immune Responses in Neutrophils from Patients with Well- and Suboptimally Controlled Asthma.

Francesca S M Tang, Gloria J Foxley, Peter G Gibson, Janette K Burgess, Katherine J Baines, Brian G Oliver

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Francesca S M TangWoolcock Institute of Medical Research, The University of Sydney, Sydney, NSW 2006, Australia ; Discipline of Pharmacology, School of Medical Sciences, Faculty of Medicine, The University of Sydney, Sydney, NSW 2006, Australia.
Gloria J FoxleyWoolcock Institute of Medical Research, The University of Sydney, Sydney, NSW 2006, Australia.
Peter G GibsonPriority Research Centre for Asthma and Respiratory Disease, The University of Newcastle, Newcastle, NSW 2308, Australia.
Janette K BurgessWoolcock Institute of Medical Research, The University of Sydney, Sydney, NSW 2006, Australia ; Discipline of Pharmacology, School of Medical Sciences, Faculty of Medicine, The University of Sydney, Sydney, NSW 2006, Australia ; Department of Pathology and Medical Biology, University of Groningen, University Medical Centre Groningen, 9713GZ Groningen, Netherlands.
Katherine J BainesPriority Research Centre for Asthma and Respiratory Disease, The University of Newcastle, Newcastle, NSW 2308, Australia.
Brian G OliverWoolcock Institute of Medical Research, The University of Sydney, Sydney, NSW 2006, Australia ; School of Life Sciences, University of Technology Sydney, Sydney, NSW 2007, Australia.
University of Sydney · AUUniversity of Newcastle Australia · AUWoolcock Institute of Medical Research · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRespiratory infections are a major cause of asthma exacerbations where neutrophilic inflammation dominates and is associated with steroid refractory asthma. Structural airway cells in asthma differ from nonasthmatics; however it is unknown if neutrophils differ. We investigated neutrophil immune responses in patients who have good (AGood) and suboptimal (ASubopt) asthma symptom control.

methodsPeripheral blood neutrophils from AGood (ACQ < 0.75, n = 11), ASubopt (ACQ > 0.75, n = 7), and healthy controls (HC) (n = 9) were stimulated with bacterial (LPS (1 μg/mL), fMLF (100 nM)), and viral (imiquimod (3 μg/mL), R848 (1.5 μg/mL), and poly I:C (10 μg/mL)) surrogates or live rhinovirus (RV) 16 (MOI1). Cell-free supernatant was collected after 1 h for neutrophil elastase (NE) and matrix metalloproteinase- (MMP-) 9 measurements or after 24 h for CXCL8 release. Results. Constitutive NE was enhanced in AGood neutrophils compared to HC. fMLF stimulated neutrophils from ASubopt but not AGood produced 50% of HC levels. fMLF induced MMP-9 was impaired in ASubopt and AGood compared to HC. fMLF stimulated CXCL8 but not MMP-9 was positively correlated with FEV1 and FEV1/FVC. ASubopt and AGood responded similarly to other stimuli.

conclusionsCirculating neutrophils are different in asthma; however, this is likely to be related to airflow limitation rather than asthma control.

Indexed as

AgedAsthmaFemaleForced Expiratory VolumeHumansImmunity, InnateInterleukin-8MaleMatrix Metalloproteinase 9Middle AgedNeutrophilsVital CapacityCXCL8 protein, humanInterleukin-8Matrix Metalloproteinase 9

Identifiers

PMID26663987
PMCPMC4667029
OpenAlexW2201469345

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.