ArticleProceedings of the National Academy of Sciences of the United States of America2015
Cell membrane deformation and bioeffects produced by tandem bubble-induced jetting flow.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Noninvasive biophysical modulations of Piezo ion channels.Microsystems & nanoengineering · 2026Review
- Tuning endothelial barrier permeability with ultrasound: a pulse-length-dependent interplay between bubble dynamics and cellular bioeffects.Ultrasonics sonochemistry · 2026Article
- Gear-like MOF microrobots for single cell mechanotransduction of microvilli.Nature communications · 2026Article
- Modeling the synergistic enhancement of drug permeation by a dual-microbubble system under ultrasonic excitation.Drug delivery · 2025Article
- Alteration of Essential Cell Function by Ultrasound-Targeted Microbubble Cavitation.Current cardiology reports · 2025Review
- Ball motion and bubble ripples in the interaction of cavitation bubble-elastic ball-curved wall.Ultrasonics sonochemistry · 2025Article
- Biomimetic Nanoparticles for Basic Drug Delivery.Pharmaceutics · 2024Review
- Facilitatory effect of low-pulse repetition frequency ultrasound on release of extracellular vesicles from cultured myotubes.Journal of medical ultrasonics (2001) · 2024Article
- Three-dimensional array of microbubbles sonoporation of cells in microfluidics.Frontiers in bioengineering and biotechnology · 2024Article
- Jetting bubbles observed by x-ray holography at a free-electron laser: internal structure and the effect of non-axisymmetric boundary conditions.Experiments in fluids · 2024Article
- Intercellular Calcium Waves and Permeability Change Induced by Vertically Deployed Surface Acoustic Waves in a Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) Monolayer.Ultrasound in medicine & biology · 2023Article
- Barrier-breaking effects of ultrasonic cavitation for drug delivery and biomarker release.Ultrasonics sonochemistry · 2023Article
- Quantifying Deformation and Migration Properties of U87 Glioma Cells Using Dielectrophoretic Forces.Biosensors · 2022Article
- Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT).IEEE transactions on medical imaging · 2022Article
- Mechanosensor Piezo1 mediates bimodal patterns of intracellular calcium and FAK signaling.The EMBO journal · 2022Article
- Review of Bubble Applications in Microrobotics: Propulsion, Manipulation, and Assembly.Micromachines · 2022Review
- Combination of ultrasound-based mechanical disruption of tumor with immune checkpoint blockade modifies tumor microenvironment and augments systemic antitumor immunity.Journal for immunotherapy of cancer · 2022Article
- The crosstalk between sonodynamic therapy and autophagy in cancer.Frontiers in pharmacology · 2022Review
- Water-Sulfuric Acid Foam as a Possible Habitat for Hypothetical Microbial Community in the Cloud Layer of Venus.Life (Basel, Switzerland) · 2021Article
- Electrochemical fabrication of TiOMaterials chemistry and physics · 2021Article
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Authors and funding
3 authors.
Funding
Abstract
Cavitation with bubble-bubble interaction is a fundamental feature in therapeutic ultrasound. However, the causal relationships between bubble dynamics, associated flow motion, cell deformation, and resultant bioeffects are not well elucidated. Here, we report an experimental system for tandem bubble (TB; maximum diameter = 50 ± 2 μm) generation, jet formation, and subsequent interaction with single HeLa cells patterned on fibronectin-coated islands (32 × 32 μm) in a microfluidic chip. We have demonstrated that pinpoint membrane poration can be produced at the leading edge of the HeLa cell in standoff distance Sd ≤ 30 μm, driven by the transient shear stress associated with TB-induced jetting flow. The cell membrane deformation associated with a maximum strain rate on the order of 10(4) s(-1) was heterogeneous. The maximum area strain ([Formula: see text]) decreased exponentially with Sd (also influenced by adhesion pattern), a feature that allows us to create distinctly different treatment outcome (i.e., necrosis, repairable poration, or nonporation) in individual cells. More importantly, our results suggest that membrane poration and cell survival are better correlated with area strain integral ([Formula: see text]) instead of [Formula: see text], which is characteristic of the response of materials under high strain-rate loadings. For 50% cell survival the corresponding area strain integral was found to vary in the range of 56 ∼ 123 μs with [Formula: see text] in the range of 57 ∼ 87%. Finally, significant variations in individual cell's response were observed at the same Sd, indicating the potential for using this method to probe mechanotransduction at the single cell level.
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