Evidence map›Paper›PMID 26663459›Full record

ArticleGenesis (New York, N.Y. : 2000)2016

Esrrb-Cre excises loxP-flanked alleles in early four-cell embryos.

Sungeun Lee, Srinivas R Pallerla, Suyeon Kim, Benjamin Shaffer, Calvin R Simerly, J Richard Chaillet, Yaacov Barak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genesis (New York, N.Y. : 2000), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Sungeun Lee *Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
Srinivas R Pallerla *Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
Suyeon KimMagee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
Benjamin ShafferMagee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
Calvin R SimerlyMagee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
J Richard ChailletMagee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
Yaacov BarakMagee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
University of Pittsburgh · USMagee-Womens Research Institute · US

Funding

UNIVERSITY OF PITTSBURGH CLINICAL AND TRANSLATIONAL SCIENCE INSTITUTE: BPCAUL1RR024153 · NCRR · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI REIS, STEVEN E · 2006 to 2011
$73.9M
Transcriptional and developmental regulation of placental fuel storage and metabP01HD069316 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI BARAK, YAACOV · 2012 to 2016
$5.0M
NCRR NIH HHS UL1 RR024153NICHD NIH HHS P01 HD069316NICHD NIH HHS P01-HD069316
6 · The paper itself

Abstract

Among transgenic mice with ubiquitous Cre recombinase activity, all strains to date excise loxP-flanked (floxed) alleles either at or before the zygote stage or at nondescript stages of development. This manuscript describes a new mouse strain, in which Cre recombinase, integrated into the Esrrb locus, efficiently excises floxed alleles in pre-implantation embryos at the onset of the four-cell stage. By enabling inactivation of genes only after the embryo has undergone two cleavages, this strain should facilitate in vivo studies of genes with essential gametic or zygotic functions. In addition, this study describes a new, highly pluripotent hybrid C57BL/6J x 129S1/SvImJ mouse embryonic stem cell line, HYB12, in which this knockin and additional targeted alleles have been generated.

Indexed as

AllelesAnimalsCell LineGene DeletionGene Transfer TechniquesIntegrasesMiceMice, Inbred C57BLMice, TransgenicReceptors, EstrogenRecombination, GeneticViral ProteinsZygoteCre recombinaseEsrrb protein, mouseIntegrasesReceptors, EstrogenViral ProteinsCre recombinaseEsrrbfour-cell embryoshybrid ES cellsknockin

Identifiers

PMID26663459
PMCPMC4724263
OpenAlexW2190733568

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.