ArticleGenesis (New York, N.Y. : 2000)2016
Generation of an estrogen receptor beta-iCre knock-in mouse.
Article in Genesis (New York, N.Y. : 2000), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Conditional ablation of Insr and Igf1r using Esr2-Cre leads to abnormal ovarian follicle development and infertility in mice†.Biology of reproduction · 2025Article
- Granulosa cell expression of Fos is critical for regulating ovulatory gene expressions in the mouse ovary.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- A Dynamic Shift in Estrogen Receptor Expression During Granulosa Cell Differentiation in the Ovary.Endocrinology · 2025Article
- Normal Ovarian Function in Subfertile Mouse withGenes · 2024Article
- Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody.Acta histochemica et cytochemica · 2022Article
- Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.Scientific reports · 2020Article
- Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation.Cell reports · 2020Article
- Principles of Genetic Engineering.Genes · 2020Review
- Lymphatic Function and Dysfunction in the Context of Sex Differences.ACS pharmacology & translational science · 2019Review
- Characterization of a BAC transgenic mouse expressing Krt19-driven iCre recombinase in its digestive organs.PloS one · 2019Article
- Estrogen Receptors: New Directions in the New Millennium.Endocrine reviews · 2018Review
- Bisphenol A and Phthalates Modulate Peritoneal Macrophage Function in Female Mice Involving SYMD2-H3K36 Dimethylation.Endocrinology · 2018Article
- Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse.Genesis (New York, N.Y. : 2000) · 2017Article
- Estrogens in Male Physiology.Physiological reviews · 2017Review
- Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
A novel knock-in mouse that expresses codon-improved Cre recombinase (iCre) under regulation of the estrogen receptor beta (Esr2) promoter was developed for conditional deletion of genes and for the spatial and/or temporal localization of Esr2 expression. ESR2 is one of two classical nuclear estrogen receptors and displays a spatiotemporal expression pattern and functions that are different from the other estrogen receptor, ESR1. A cassette was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker. This construct was used to insert iCre in front of the endogenous start codon of the Esr2 gene of a C57BL/6J embryonic stem cell line via homologous recombination. Resulting Esr2-iCre mice were bred with ROSA26-lacZ and Ai9-RFP reporter mice to visualize cells of functional iCre expression. Strong expression was observed in the ovary, the pituitary, the interstitium of the testes, the head and tail but not body of the epididymis, skeletal muscle, the coagulation gland (anterior prostate), the lung, and the preputial gland. Additional diffuse or patchy expression was observed in the cerebrum, the hypothalamus, the heart, the adrenal gland, the colon, the bladder, and the pads of the paws. Overall, Esr2-iCre mice will serve as a novel line for conditionally ablating genes in Esr2-expressing tissues, identifying novel Esr2-expressing cells, and differentiating the functions of ESR2 and ESR1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.