ArticleThe Journal of biological chemistry2016
Phosphatidylethanolamine Is a Key Regulator of Membrane Fluidity in Eukaryotic Cells.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 207 papers, 1 of them a synthesis that pooled it.
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Who cites it
207 citing papers in PubMed, 1 synthesis or guideline pooled it, 388 citations in OpenAlex.
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- Association of Different Host Diets with the Nutritional Composition of the Fall Webworm,Insects · 2026Article
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- Targeted lipidomics between hPheo1 and SDHB KD cells reveal changes in bioactive lipids and PKC with polyamine pathway inhibition.Physiological reports · 2026Article
- Active regulation of intrinsic curvature by eukaryotic phospholipid metabolism.Cell reports. Physical science · 2026Article
- Hoi1 targets BLTP2 to ER-PM contact sites to regulate lipid homeostasis.The Journal of cell biology · 2026Article
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- Enhancement of Disease Resistance in Pengze Crucian Carp (Metabolites · 2026Article
- Phosphatidylserine Decarboxylase Regulates Retinal Ganglion Cell Neurite Outgrowth with Altered Somal Membrane Fluidics and Mitochondrial Morphology.Biomolecules · 2026Article
- Lipid Matters: How Herbicidal Ionic Liquids Engage with Membranes.The journal of physical chemistry. B · 2026Article
- Overcoming divalent cation sensitivity is not the only challenge for functional study of ABC transporters within polymer lipid particles.Bioscience reports · 2026Article
- Age-Driven Lipid Remodeling Activates Lysosome-Mediated Plasma Membrane Repair.Research square · 2026Article
- Insect Lipids: Structure, Classification and Function.Advances in experimental medicine and biology · 2026Review
- Three phosphatidylcholines as a potential early-diagnostic signature for hyperglycemia in preschool-aged children: a cross-sectional pilot study.Frontiers in endocrinology · 2026Article
147 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adequate membrane fluidity is required for a variety of key cellular processes and in particular for proper function of membrane proteins. In most eukaryotic cells, membrane fluidity is known to be regulated by fatty acid desaturation and cholesterol, although some cells, such as insect cells, are almost devoid of sterol synthesis. We show here that insect and mammalian cells present similar microviscosity at their respective physiological temperature. To investigate how both sterols and phospholipids control fluidity homeostasis, we quantified the lipidic composition of insect SF9 and mammalian HEK 293T cells under normal or sterol-modified condition. As expected, insect cells show minimal sterols compared with mammalian cells. A major difference is also observed in phospholipid content as the ratio of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) is inverted (4 times higher in SF9 cells). In vitro studies in liposomes confirm that both cholesterol and PE can increase rigidity of the bilayer, suggesting that both can be used by cells to maintain membrane fluidity. We then show that exogenously increasing the cholesterol amount in SF9 membranes leads to a significant decrease in PE:PC ratio whereas decreasing cholesterol in HEK 293T cells using statin treatment leads to an increase in the PE:PC ratio. In all cases, the membrane fluidity is maintained, indicating that both cell types combine regulation by sterols and phospholipids to control proper membrane fluidity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.