ArticleThe Journal of biological chemistry2016
The Transcription Factor THO Promotes Transcription Initiation and Elongation by RNA Polymerase I.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2.eLife · 2021Article
- NETSeq reveals heterogeneous nucleotide incorporation by RNA polymerase I.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels.Journal of industrial microbiology & biotechnology · 2017Article
- Transcription factors that influence RNA polymerases I and II: To what extent is mechanism of action conserved?Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Although ribosomal RNA represents the majority of cellular RNA, and ribosome synthesis is closely connected to cell growth and proliferation rates, a complete understanding of the factors that influence transcription of ribosomal DNA is lacking. Here, we show that the THO complex positively affects transcription by RNA polymerase I (Pol I). We found that THO physically associates with the rDNA repeat and interacts genetically with Pol I transcription initiation factors. Pol I transcription in hpr1 or tho2 null mutants is dramatically reduced to less than 20% of the WT level. Pol I occupancy of the coding region of the rDNA in THO mutants is decreased to ~50% of WT level. Furthermore, although the percentage of active rDNA repeats remains unaffected in the mutant cells, the overall rDNA copy number increases ~2-fold compared with WT. Together, these data show that perturbation of THO function impairs transcription initiation and elongation by Pol I, identifying a new cellular target for the conserved THO complex.
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Registered trials
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