ArticleThe Journal of biological chemistry2016
DNA Gyrase Is the Target for the Quinolone Drug Ciprofloxacin in Arabidopsis thaliana.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 70 citations in OpenAlex.
- Triphenylphosphonium is an effective targeting moiety for plant mitochondria.The New phytologist · 2025Article
- Antibacterial Activity, Phytochemical AnalysisIndian journal of microbiology · 2025Article
- Population pharmacokinetics of ciprofloxacin in newborns with early onset neonatal sepsis and suspected meningitis.Pediatric research · 2024Article
- TALE-based organellar genome editing and gene expression in plants.Plant cell reports · 2024Review
- Survey of probable synergism between melittin and ciprofloxacin, rifampicin, and chloramphenicol against multidrug-resistantFrontiers in microbiology · 2024Article
- Overview of Direct and Indirect Effects of Antibiotics on Terrestrial Organisms.Antibiotics (Basel, Switzerland) · 2023Review
- Article
- Repurposed inhibitor of bacterial dihydrodipicolinate reductase exhibits effective herbicidal activity.Communications biology · 2023Article
- DNA Gyrase as a Target for Quinolones.Biomedicines · 2023Review
- Molecular mechanism of topoisomerase poisoning by the peptide antibiotic albicidin.Nature catalysis · 2023Article
- Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in Arabidopsis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- New chalcone derivatives as potential antimicrobial and antioxidant agent.Scientific reports · 2021Article
- Inhibitory Compounds Targeting Plasmodium falciparum Gyrase B.Antimicrobial agents and chemotherapy · 2021Article
- Herbicidal activity of fluoroquinolone derivatives.Plant direct · 2021Article
- Cytosolic CTP Production Limits the Establishment of Photosynthesis inFrontiers in plant science · 2021Article
- Structure-Function Analysis Reveals the Singularity of Plant Mitochondrial DNA Replication Components: A Mosaic and Redundant System.Plants (Basel, Switzerland) · 2019Review
- Albicidin, a potent DNA gyrase inhibitor with clinical potential.The Journal of antibiotics · 2019Review
- Ecotoxicological effects and accumulation of ciprofloxacin in Eichhornia crassipes under hydroponic conditions.Environmental science and pollution research international · 2019Article
- Current Status and Future Prospects in Herbicide Discovery.Plants (Basel, Switzerland) · 2019Review
- Single-nucleotide-resolution mapping of DNA gyrase cleavage sites across the Escherichia coli genome.Nucleic acids research · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
The Arabidopsis thaliana genome contains four genes that were originally annotated as potentially encoding DNA gyrase: ATGYRA, ATGYRB1, ATGYRB2, and ATGYRB3. Although we subsequently showed that ATGYRB3 does not encode a gyrase subunit, the other three genes potentially encode subunits of a plant gyrase. We also showed evidence for the existence of supercoiling activity in A. thaliana and that the plant is sensitive to quinolone and aminocoumarin antibiotics, compounds that target DNA gyrase in bacteria. However, it was not possible at that time to show whether the A. thaliana genes encoded an active gyrase enzyme, nor whether that enzyme is indeed the target for the quinolone and aminocoumarin antibiotics. Here we show that an A. thaliana mutant resistant to the quinolone drug ciprofloxacin has a point mutation in ATGYRA. Moreover we show that, as in bacteria, the quinolone-sensitive (wild-type) allele is dominant to the resistant gene. Further we have heterologously expressed ATGYRA and ATGYRB2 in a baculovirus expression system and shown supercoiling activity of the partially purified enzyme. Expression/purification of the quinolone-resistant A. thaliana gyrase yields active enzyme that is resistant to ciprofloxacin. Taken together these experiments now show unequivocally that A. thaliana encodes an organelle-targeted DNA gyrase that is the target of the quinolone drug ciprofloxacin; this has important consequences for plant physiology and the development of herbicides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.