ArticleThe Plant journal : for cell and molecular biology2016
A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell type-specific functional genomics in Arabidopsis.
Article in The Plant journal : for cell and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 171 citations in OpenAlex.
- TurboID-based proteomic profiling reveals proxitome of the IRT1 metal transporter and new insight into IRT1 endocytosis.The New phytologist · 2026Article
- SRF3 couples cellular iron homeostasis and defense responses through ubiquitin-dependent nano-organization.Science advances · 2026Article
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- Brassinosteroids control cell proliferation in the lateral root cap of the Arabidopsis root.EMBO reports · 2026Article
- Non-linear stress-softening of peptidoglycan mediates bacterial cell shape homeostasis.Current biology : CB · 2026Article
- The Volatile Pentadecane From Bacillus Alleviates Plant Iron Deficiency Through Activating the Reduction-Based Fe Uptake System.Plant biotechnology journal · 2026Article
- mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.Plant physiology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A new use of Agrobacterium plant growth regulator genes for plant bioengineering.Frontiers in plant science · 2026Article
- An RbohC and RbohE-mediated ROS oscillatory circuit regulates root cap turnover in Arabidopsis.iScience · 2025Article
- Cell wall-derived mechanical signals control cell growth and division during root development.Science advances · 2025Article
- Genetically Reprogramming Crops and Rhizobacteria for Nutritional Iron Biofortification.ACS synthetic biology · 2025Review
- The epidermis coordinates multi-scale symmetry breaking in chiral root growth.Nature communications · 2025Article
- Canonical tyrosine-based motifs are required for constitutive endocytosis and polarity of IRT1 and contribute to metal uptake.The Plant journal : for cell and molecular biology · 2025Article
- Abscisic acid and GIGANTEA signalling converge to regulate the recruitment of CONSTANS to the FT promoter and activate floral transition.Journal of experimental botany · 2025Article
- Broad application of the ALFA tagging technology for in planta nanobody-based imaging and biochemical characterization of plant proteins.Plant communications · 2025Article
- Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis.Nature communications · 2025Article
- Four ZIPs contribute to Zn, Fe, Cu and Mn acquisition at the outer root domain.PLoS genetics · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- SeedSeg: image-based transgenic seed counting for segregation analysis of T-DNA loci.Plant methods · 2025Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 8 institutions in 2 countries.
Funding
Abstract
Multicellular organisms are composed of many cell types that acquire their specific fate through a precisely controlled pattern of gene expression in time and space dictated in part by cell type-specific promoter activity. Understanding the contribution of highly specialized cell types in the development of a whole organism requires the ability to isolate or analyze different cell types separately. We have characterized and validated a large collection of root cell type-specific promoters and have generated cell type-specific marker lines. These benchmarked promoters can be readily used to evaluate cell type-specific complementation of mutant phenotypes, or to knockdown gene expression using targeted expression of artificial miRNA. We also generated vectors and characterized transgenic lines for cell type-specific induction of gene expression and cell type-specific isolation of nuclei for RNA and chromatin profiling. Vectors and seeds from transgenic Arabidopsis plants will be freely available, and will promote rapid progress in cell type-specific functional genomics. We demonstrate the power of this promoter set for analysis of complex biological processes by investigating the contribution of root cell types in the IRT1-dependent root iron uptake. Our findings revealed the complex spatial expression pattern of IRT1 in both root epidermis and phloem companion cells and the requirement for IRT1 to be expressed in both cell types for proper iron homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.