ArticleThe Plant journal : for cell and molecular biology2016
Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2.
Article in The Plant journal : for cell and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed, 123 citations in OpenAlex.
- Machine learning-assisted directed evolution of plant Rubisco.Science advances · 2026Article
- Chloroplast genome editing of Rubisco boosts photosynthesis and plant growth.Nature communications · 2026Article
- PtCP1 Is an Extraplastidial Cysteine Protease Involved in Leaf Protein Degradation ofPlants (Basel, Switzerland) · 2026Article
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- High-Throughput Detection of Cyanobacterial Form I Rubisco Assembly.ACS synthetic biology · 2026Article
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Engineering Spatial Control of Bacterial Organelles.bioRxiv : the preprint server for biology · 2025Article
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Engineering COPlant communications · 2025Article
- Review
- Discussion of the possibility of increasing the efficiency of photosynthesis using genetic and metabolic engineering methods.Biodesign research · 2025Review
- Towards assembling functional cyanobacterial β-carboxysomes in Oryza sativa chloroplasts.Functional & integrative genomics · 2025Article
- Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).Plant cell reports · 2024Article
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- Transgenic strategies to improve the thermotolerance of photosynthesis.Photosynthesis research · 2023Review
- Towards engineering a hybrid carboxysome.Photosynthesis research · 2023Article
- Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis.Nature communications · 2023Article
- Producing fast and active Rubisco in tobacco to enhance photosynthesis.The Plant cell · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Introducing a carbon-concentrating mechanism and a faster Rubisco enzyme from cyanobacteria into higher plant chloroplasts may improve photosynthetic performance by increasing the rate of CO2 fixation while decreasing losses caused by photorespiration. We previously demonstrated that tobacco plants grow photoautotrophically using Rubisco from Synechococcus elongatus, although the plants exhibited considerably slower growth than wild-type and required supplementary CO2 . Because of concerns that vascular plant assembly factors may not be adequate for assembly of a cyanobacterial Rubisco, prior transgenic plants included the cyanobacterial chaperone RbcX or the carboxysomal protein CcmM35. Here we show that neither RbcX nor CcmM35 is needed for assembly of active cyanobacterial Rubisco. Furthermore, by altering the gene regulatory sequences on the Rubisco transgenes, cyanobacterial Rubisco expression was enhanced and the transgenic plants grew at near wild-type growth rates, although still requiring elevated CO2 . We performed detailed kinetic characterization of the enzymes produced with and without the RbcX and CcmM35 cyanobacterial proteins. These transgenic plants exhibit photosynthetic characteristics that confirm the predicted benefits of introduction of non-native forms of Rubisco with higher carboxylation rate constants in vascular plants and the potential nitrogen-use efficiency that may be achieved provided that adequate CO2 is available near the enzyme.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.