ArticleTransboundary and emerging diseases2017
Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.
Article in Transboundary and emerging diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Innovations, Applications, and Future Trends in Veterinary Diagnostic Technologies.Transboundary and emerging diseases · 2026Review
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Challenges and Lessons Learned from a Field Trial on the Understanding of the Porcine Respiratory Disease Complex.Vaccines · 2025Article
- Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2.BMC veterinary research · 2025Article
- Metagenomics to Identify Viral Communities Associated with Porcine Respiratory Disease Complex in Tibetan Pigs in the Tibetan Plateau, China.Pathogens (Basel, Switzerland) · 2024Article
- Article
- Multiplex detection and identification of viral, bacterial, and protozoan pathogens in human blood and plasma using an expanded high-density resequencing microarray platform.Frontiers in molecular biosciences · 2024Article
- Recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge.BMC veterinary research · 2023Article
- Associations between Pleurisy and the Main Bacterial Pathogens of the Porcine Respiratory Diseases Complex (PRDC).Animals : an open access journal from MDPI · 2023Article
- A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs.Veterinary research · 2023Article
- Porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus alone or associated are frequent intralesional detected viruses in porcine respiratory disease complex cases in Northern Italy.Frontiers in veterinary science · 2023Article
- Detection of Porcine Circovirus Type 2a andVeterinary sciences · 2022Article
- The Role of Pathology in the Diagnosis of Swine Respiratory Disease.Veterinary sciences · 2021Review
- Seasonal Variation in Prevalence ofPathogens (Basel, Switzerland) · 2021Article
- Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing.Veterinary world · 2020Article
- Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases.The Journal of veterinary medical science · 2020Article
- Article
- A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens.Journal of virological methods · 2018Article
- Viral communities associated with porcine respiratory disease complex in intensive commercial farms in Sichuan province, China.Scientific reports · 2018Article
- A multiplex reverse transcription PCR and automated electronic microarray assay for detection and differentiation of seven viruses affecting swine.Transboundary and emerging diseases · 2018Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine respiratory disease complex (PRDC) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. No simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with PRDC. Furthermore, the detection of most of the bacterial pathogens implicated in PRDC currently requires time-consuming culture-based methods that can take several days to obtain results. In this study, a novel prototype automated microarray that integrates and automates all steps of post-PCR microarray processing for the simultaneous detection and typing of eight bacteria and viruses commonly associated with PRDC is described along with associated multiplex reverse transcriptase PCR. The user-friendly assay detected and differentiated between four viruses [porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus, porcine circovirus type 2, porcine respiratory corona virus], four bacteria (Mycoplasma hyopneumoniae, Pasteurella multocida, Salmonella enterica serovar Choleraesuis, Streptococcus suis), and further differentiated between type 1 and type 2 PRRSV as well as toxigenic and non-toxigenic P. multocida. The assay accurately identified and typed a panel of 34 strains representing the eight targeted pathogens and was negative when tested with 34 relevant and/or closely related non-target bacterial and viral species. All targets were also identified singly or in combination in a panel of clinical lung samples and/or experimentally inoculated biological material.
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