ArticlePlant physiology2016
Arabidopsis RhoGDIs Are Critical for Cellular Homeostasis of Pollen Tubes.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Effects of fluorescent tags and activity status on the membrane localization of ROP GTPases.Plant signaling & behavior · 2024Article
- Two subtypes of GTPase-activating proteins coordinate tip growth and cell size regulation in Physcomitrium patens.Nature communications · 2023Article
- Phosphorylation of RhoGDI1, a Rho GDP dissociation inhibitor, regulates root hair development inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Signaling network controlling ROP-mediated tip growth in Arabidopsis and beyond.Plant communications · 2023Review
- Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI.Plants (Basel, Switzerland) · 2022Article
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Cross-talk between clathrin-dependent post-Golgi trafficking and clathrin-mediated endocytosis in Arabidopsis root cells.The Plant cell · 2021Article
- Function of membrane domains in rho-of-plant signaling.Plant physiology · 2021Review
- Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth.The protein journal · 2021Article
- The canonical α-SNAP is essential for gametophytic development in Arabidopsis.PLoS genetics · 2021Article
- Rho GTPase ROP1 Interactome Analysis Reveals Novel ROP1-Associated Pathways for Pollen Tube Polar Growth in Arabidopsis.International journal of molecular sciences · 2020Article
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- COPII Components Sar1b and Sar1c Play Distinct Yet Interchangeable Roles in Pollen Development.Plant physiology · 2020Article
- Exocytosis and endocytosis: coordinating and fine-tuning the polar tip growth domain in pollen tubes.Journal of experimental botany · 2020Review
- ArabidopsisThe Plant cell · 2020Article
- Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg.Plants (Basel, Switzerland) · 2019Review
- Systematic survey of the function of ROP regulators and effectors during tip growth in the moss Physcomitrella patens.Journal of experimental botany · 2019Article
- A Tonoplast-Associated Calcium-Signaling Module Dampens ABA Signaling during Stomatal Movement.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhos of plants (ROPs) play a key role in plant cell morphogenesis, especially in tip-growing pollen tubes and root hairs, by regulating an array of intracellular activities such as dynamic polymerization of actin microfilaments. ROPs are regulated by guanine nucleotide exchange factors (RopGEFs), GTPase activating proteins (RopGAPs), and guanine nucleotide dissociation inhibitors (RhoGDIs). RopGEFs and RopGAPs play evolutionarily conserved function in ROP signaling. By contrast, although plant RhoGDIs regulate the membrane extraction and cytoplasmic sequestration of ROPs, less clear are their positive roles in ROP signaling as do their yeast and metazoan counterparts. We report here that functional loss of all three Arabidopsis (Arabidopsis thaliana) GDIs (tri-gdi) significantly reduced male transmission due to impaired pollen tube growth in vitro and in vivo. We demonstrate that ROPs were ectopically activated at the lateral plasma membrane of the tri-gdi pollen tubes. However, total ROPs were reduced posttranslationally in the tri-gdi mutant, resulting in overall dampened ROP signaling. Indeed, a ROP5 mutant that was unable to interact with GDIs failed to induce growth, indicating the importance of the ROP-GDI interaction for ROP signaling. Functional loss of GDIs impaired cellular homeostasis, resulting in excess apical accumulation of wall components in pollen tubes, similar to that resulting from ectopic phosphatidylinositol 4,5-bisphosphate signaling. GDIs and phosphatidylinositol 4,5-bisphosphate may antagonistically coordinate to maintain cellular homeostasis during pollen tube growth. Our results thus demonstrate a more complex role of GDIs in ROP-mediated pollen tube growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.