Evidence map›Paper›PMID 26660729›Full record

ArticleTransgenic research2016

A transgenic, visual screenable marker for soybean seeds.

Lisa Kanizay, Thomas Jacobs, C Nathan Hancock

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Lisa KanizayCenter for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.
Thomas JacobsCenter for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.
C Nathan HancockDepartment of Biology and Geology, University of South Carolina Aiken, 471 University Parkway, Aiken, SC, 29801, USA. nathanh@usca.edu.
Applied Genetic Technologies (United States) · USUniversity of Georgia · USUniversity of South Carolina Aiken · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Most soybean cultivars produce buff colored seeds due to a seed coat specific siRNA mechanism. This phenomenon is specifically limited to the seed coat and produces a strong visual effect, thus, a strategy to evade the silencing was used to produce a maternal transgenic marker for soybeans. Expression of a rice chalcone synthase transgene with little DNA sequence homology to the soybean siRNAs resulted in dark colored seed coats. This phenotype is the result of anthocyanin pigment production and does not appear to affect other tissues. This novel approach for producing an easily scored transgenic marker for soybean will facilitate high-throughput screening and analysis of transgenic soybean.

Indexed as

AcyltransferasesBiomarkersGene Expression Regulation, PlantGene SilencingGlycine maxOryzaPlants, Genetically ModifiedRNA, Small InterferingSeedsAcyltransferasesBiomarkersflavanone synthetaseRNA, Small InterferingChalcone synthaseRNA silencingSeed coatSoybeanVisual screening

Identifiers

PMID26660729
OpenAlexW2196391887

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.