ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2016
MicroRNA regulation in heart and skeletal muscle over the freeze-thaw cycle in the freeze tolerant wood frog.
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 45 citations in OpenAlex.
- The transcriptomics and differential gene expression of freezing and thawing in a freeze-tolerant vertebrate.BMC genomics · 2026Article
- Environmental Changes Drives the Transcriptome and Gene Regulation Plasticity During Sea Lice Infestation.Marine biotechnology (New York, N.Y.) · 2025Article
- Epigenetic Regulation by Histone Methylation and Demethylation in Freeze-Tolerant Frog Kidney.Cell biochemistry and function · 2024Article
- Role of MicroRNAs in Extreme Animal Survival Strategies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Muscles in Winter: The Epigenetics of Metabolic Arrest.Epigenomes · 2021Review
- MicroRNA Cues from Nature: A Roadmap to Decipher and Combat Challenges in Human Health and Disease?Cells · 2021Review
- Hepatic transcriptome of the freeze-tolerant Cope's gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation and freezing.BMC genomics · 2020Article
- Waking the sleeping dragon: gene expression profiling reveals adaptive strategies of the hibernating reptile Pogona vitticeps.BMC genomics · 2019Article
- Micromanaging freeze tolerance: the biogenesis and regulation of neuroprotective microRNAs in frozen brains.Cellular and molecular life sciences : CMLS · 2018Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The North American wood frog, Rana sylvatica, is one of just a few anuran species that tolerates whole body freezing during the winter and has been intensely studied to identify the biochemical adaptations that support freeze tolerance. Among these adaptations is the altered expression of many genes, making freeze-responsive changes to gene regulatory mechanisms a topic of interest. The present study focuses on the potential involvement of microRNAs as one such regulatory mechanism and aims to better understand freeze/thaw stress-induced microRNA responses in the freeze-tolerant wood frog. Using quantitative PCR, relative levels of 53 microRNAs were measured in heart and skeletal muscle of control, 24 h frozen, and 8 h thawed frogs. MicroRNAs showed tissue specific expression patterns: 21 microRNAs decreased in the heart during thawing, whereas 16 microRNAs increased during freezing stress in skeletal muscle. These findings suggest that select genes may be activated and suppressed in heart and skeletal muscle, respectively, in response to freezing. Bioinformatics analysis using the DIANA miRPath program (v.2.0) predicted that the differentially expressed microRNAs may collectively regulate tissue-specific cellular pathways to promote survival of wood frogs undergoing freezing and thawing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.