ArticlePhysiological reports2015
Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells.
Article in Physiological reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Impact of aerobic exercise and fatty acid supplementation on global and gene-specific DNA methylation.Epigenetics · 2019Trial
- Acute Strenuous Exercise Induces an Imbalance on Histone H4 Acetylation/Histone Deacetylase 2 and Increases the Proinflammatory Profile of PBMC of Obese Individuals.Oxidative medicine and cellular longevity · 2017Trial
- DNA Methylation in the Adaptive Response to Exercise.Sports medicine (Auckland, N.Z.) · 2024Review
- Hypomethylation in promoters of PGC-1α involved in exercise-driven skeletal muscular alterations in old age.Open life sciences · 2024Article
- Article
- No dynamic changes in the expression of genes related to the epigenetic mechanism during acute exercise.Journal of applied genetics · 2023Article
- Potential Role of Chronic Physical Exercise as a Treatment in the Development of Vitiligo.Frontiers in physiology · 2022Article
- Physical Activity and DNA Methylation in Humans.International journal of molecular sciences · 2021Review
- Exercise, redox homeostasis and the epigenetic landscape.Redox biology · 2020Review
- The Influence of Physical Activity and Epigenomics On Cognitive Function and Brain Health in Breast Cancer.Frontiers in aging neuroscience · 2020Review
- Adipose Tissue Senescence and Inflammation in Aging is Reversed by the Young Milieu.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019Article
- Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells-A Pilot Study.Genes · 2019Article
- Effects of age and sex on epigenetic modification induced by an acute physical exercise: Retraction.Medicine · 2018Article
- Effects of age and sex on epigenetic modification induced by an acute physical exercise.Medicine · 2017Observational
- Exercise and the Skeletal Muscle Epigenome.Cold Spring Harbor perspectives in medicine · 2017Review
- Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells.Physiological reports · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is modifiable by acute and chronic exercise. DNA methyltransferases (DNMT) catalyze this process; however, there is a lack of literature concerning the specific mechanisms by which exercise-induced modifications occur. Interleukin 6 (IL-6) stimulation of various cell lines has been shown to augment DNMT expression and nuclear translocation, which suggests a possible pathway by which exercise is able to elicit changes in epigenetic enzymes. The present study sought to elucidate the response of the de novo methyltransferases DNMT3A and DNMT3B to circulatory factors found in plasma isolated from whole blood before and after 120-min of treadmill running at an intensity of 60% of individual velocity at V˙O2max (vV˙O2max) interspersed with 30-sec sprints at 90% of vV˙O2max every 10-min. Peripheral blood mononuclear cells (PBMCs) isolated from a resting participant were incubated with plasma isolated from exercising participants (n = 10) or recombinant IL-6 (rIL-6), followed by nuclear protein extraction and quantification of DNMT3A and DNMT3B concentrations. Nuclear concentrations of DNMT3B significantly decreased following the experimental protocol (P = 0.03), with no change observed in DNMT3A (P = 0.514).Various concentrations of rIL-6 caused an elevation in both DNMT3A and DNMT3B nuclear concentration compared with the blank control. The conflicting results between exercising and rIL-6 conditions suggests that IL-6 does regulate DNMT nuclear transport, however, other plasma mediators may also exert significant influence on the nuclear concentrations of these enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.