Evidence map›Paper›PMID 26660475›Full record

ArticlePloS one2015

Packed Bed Bioreactor for the Isolation and Expansion of Placental-Derived Mesenchymal Stromal Cells.

Michael J Osiecki, Thomas D Michl, Betul Kul Babur, Mahboubeh Kabiri, Kerry Atkinson, William B Lott, Hans J Griesser, Michael R Doran

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 44 citations in OpenAlex.

  1. Review
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  8. Development of surface functionalization strategies for 3D-printed polystyrene constructs.Journal of biomedical materials research. Part B, Applied biomaterials · 2019
    Article
  9. The Evolution of Polystyrene as a Cell Culture Material.Tissue engineering. Part B, Reviews · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Michael J OsieckiInstitute of Health and Biomedical Innovation, Queensland University of Technology at the Translational Research Institute, Brisbane, Queensland, Australia.
Thomas D MichlIan Wark Research Institute, University of South Australia. Adelaide, South Australia, Australia.
Betul Kul BaburInstitute of Health and Biomedical Innovation, Queensland University of Technology at the Translational Research Institute, Brisbane, Queensland, Australia.
Mahboubeh KabiriDepartment of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Kerry AtkinsonInstitute of Health and Biomedical Innovation, Queensland University of Technology at the Translational Research Institute, Brisbane, Queensland, Australia.
William B LottInstitute of Health and Biomedical Innovation, Queensland University of Technology at the Translational Research Institute, Brisbane, Queensland, Australia.
Hans J GriesserMawson Institute, University of South Australia. Adelaide, South Australia, Australia.
Michael R DoranInstitute of Health and Biomedical Innovation, Queensland University of Technology at the Translational Research Institute, Brisbane, Queensland, Australia.
Queensland University of Technology · AUTranslational Research Institute · AUUniversity of South Australia · AUUniversity of Queensland · AUUniversity of Tehran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pellets that were air plasma treated to endow them with a surface chemistry similar to traditional tissue culture plastic. The packed bed was encased within a gas permeable shell to decouple the medium nutrient supply and gas exchange. This enabled a significant reduction in medium flow rates, thus reducing shear and even facilitating single pass medium exchange. The system was optimised in a small-scale bioreactor format (160 cm2) with murine-derived green fluorescent protein-expressing MSCs, and then scaled-up to a 2800 cm2 format. We demonstrated that placental derived MSCs could be isolated directly within the bioreactor and subsequently expanded. Our results demonstrate that the closed system large-scale packed bed bioreactor is an effective and scalable tool for large-scale isolation and expansion of MSCs.

Indexed as

BioreactorsAdipogenesisAnimalsBone Marrow CellsCell Culture TechniquesCell DifferentiationChondrogenesisFemaleGreen Fluorescent ProteinsHumansMesenchymal Stem CellsMiceOsteogenesisOxygenPlacentaPolystyrenesGreen Fluorescent ProteinsOxygenPolystyrenes

Identifiers

PMID26660475
PMCPMC4687640
OpenAlexW2211594303

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.