ArticleLab on a chip2016
Surface-tension driven open microfluidic platform for hanging droplet culture.
Article in Lab on a chip, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Microfluidics for Blood Disorders and Hematological Disease Monitoring and Modeling.International journal of molecular sciences · 2026Review
- EnvZ/OmpR-driven cooperative behavior promotes cefiderocol resistance in a hanging-droplet evolution system.Science advances · 2026Article
- Role of Exosomes in Cardiovascular Disease: A Key Regulator of Intercellular Communication in Cardiomyocytes.ACS omega · 2025Review
- Open Microfluidic Cell Culture in Hydrogels Enabled by 3D-Printed Molds.Bioengineering (Basel, Switzerland) · 2025Article
- Spheroid-Exosome-Based Bioprinting Technology in Regenerative Medicine.Journal of functional biomaterials · 2024Review
- On the design and fabrication of nanoliter-volume hanging drop networks.Microsystems & nanoengineering · 2024Article
- Recent Advances in Dielectrophoretic Manipulation and Separation of Microparticles and Biological Cells.Biosensors · 2024Review
- Small extracellular vesicles purification and scale-up.Frontiers in immunology · 2024Review
- Application of microfluidic technology based on surface-enhanced Raman scattering in cancer biomarker detection: A review.Journal of pharmaceutical analysis · 2023Review
- Various Three-Dimensional Culture Methods and Cell Types for Exosome Production.Tissue engineering and regenerative medicine · 2023Review
- Miniaturizing chemistry and biology using droplets in open systems.Nature reviews. Chemistry · 2023Review
- Interfacial tension driven open droplet microfluidics.Advanced materials interfaces · 2023Article
- Review
- Non-small cell lung carcinoma spheroid models in agarose microwells for drug response studies.Lab on a chip · 2022Article
- Flipped Well-Plate Hanging-Drop Technique for Growing Three-Dimensional Tumors.Frontiers in bioengineering and biotechnology · 2022Article
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Cutting and Bonding ParafilmCellular and molecular bioengineering · 2021Article
- Review
- A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.Frontiers in bioengineering and biotechnology · 2021Article
- Organotypic Models in Drug Development "Tumor Models and Cancer Systems Biology for the Investigation of Anticancer Drugs and Resistance Development".Handbook of experimental pharmacology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The hanging droplet technique for three-dimensional tissue culture has been used for decades in biology labs, with the core technology remaining relatively unchanged. Recently microscale approaches have expanded the capabilities of the hanging droplet method, making it more user-friendly. We present a spontaneously driven, open hanging droplet culture platform to address many limitations of current platforms. Our platform makes use of two interconnected hanging droplet wells, a larger well where cells are cultured and a smaller well for user interface via a pipette. The two-well system results in lower shear stress in the culture well during fluid exchange, enabling shear sensitive or non-adherent cells to be cultured in a droplet. The ability to perform fluid exchanges in-droplet enables long-term culture, treatment, and characterization without disruption of the culture. The open well format of the platform was utilized to perform time-dependent coculture, enabling culture configurations with bone tissue scaffolds and cells grown in suspension. The open nature of the system allowed the direct addition or removal of tissue over the course of an experiment, manipulations that would be impractical in other microfluidic or hanging droplet culture platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.