Evidence map›Paper›PMID 26659801›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

In Situ Sodium Bisulfite Modification of Genomic DNA from Microdissected Melanoma Paraffin-Embedded Archival Tissues.

Diego M Marzese, Sharon K Huang, Dave S B Hoon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Experimental and therapeutic medicine · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Diego M MarzeseDepartment of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA.
Sharon K HuangDepartment of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA.
Dave S B HoonDepartment of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA. hoon@jwci.org.
Molecular Oncology (United States) · USSaint John's Health Center · US

Funding

SURGICAL ONCOLOGY WITH COMBINATION THERMO-CHEMOTHERAPYP01CA029605 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI COCHRAN, ALISTAIR JOHN · 1985 to 2013
$75.2M
Identification of Melanoma Brain Metastasis Tumor BiomarkersR01CA167967 · NCI · SAINT JOHN'S CANCER INSTITUTE · PI HOON, DAVE S B · 2013 to 2017
$1.9M
NCI NIH HHS P01 CA029605NCI NIH HHS R01 CA167967
6 · The paper itself

Abstract

DNA methylation analysis of paraffin-embedded archival tumor tissues (PEAT) is important in clinical and translational research studies. Efficient identification and isolation of homogeneous cell population, optimal DNA extraction, and sodium bisulfite modification (SBM) are essential, particularly in small tumor lesions. Laser capture microdissection (LCM) coupled with an in situ SBM improves the specificity, through histopathology accuracy, and the amount of genomic DNA modified for downstream methylation assays.

Indexed as

DNA methylationLaser capture microdissection (LCM)MelanomaSodium bisulfite modification (SBM)

Identifiers

PMID26659801
PMCPMC5329140
OpenAlexW2412510659

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.