Evidence map›Paper›PMID 26659793›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Aggregate and Microcarrier Cultures of Human Pluripotent Stem Cells in Stirred-Suspension Systems.

Preeti Ashok, Yongjia Fan, Mahboubeh R Rostami, Emmanuel S Tzanakakis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Preeti AshokDepartment of Chemical and Biological Engineering, Tufts University, 4 Colby Street, 276A, Medford, MA, 02155, USA.
Yongjia FanDepartment of Chemical and Biological Engineering, Tufts University, 4 Colby Street, 276A, Medford, MA, 02155, USA.
Mahboubeh R RostamiDepartment of Chemical and Biological Engineering, Tufts University, 4 Colby Street, 276A, Medford, MA, 02155, USA.
Emmanuel S TzanakakisDepartment of Chemical and Biological Engineering, Tufts University, 4 Colby Street, 276A, Medford, MA, 02155, USA. Emmanuel.Tzanakakis@tufts.edu.
Tufts University · USTufts Medical Center · US

Funding

Bioprocess for cardiac cell generation from human induced pluripotent stem cellsR01HL103709 · NHLBI · TUFTS UNIVERSITY MEDFORD · PI TZANAKAKIS, EMMANOUHL · 2011 to 2014
$1.5M
NHLBI NIH HHS R01 HL103709
6 · The paper itself

Abstract

Pluripotent stem cells can differentiate to any cell type and contribute to damaged tissue repair and organ function reconstitution. The scalable culture of pluripotent stem cells is essential to furthering the use of stem cell products in a wide gamut of applications such as screening of candidate drugs and cell replacement therapies. Human stem cell cultivation in stirred-suspension vessels enables the expansion of stem cells and the generation of differentiated progeny in quantities suitable for use in animal models and clinical studies. We describe methods of culturing human pluripotent stem cells in spinner flasks either as aggregates or on microcarriers. Techniques for assessing the quality of the culture and characterizing the cells based on the presentation of pertinent markers are also presented. Spinner flask culture with its relatively low capital and operating costs is appealing to laboratories interested in scaling up their production of stem/progenitor cells.

Indexed as

Cell AggregationCell Culture TechniquesCell DifferentiationCell LineEndodermFlow CytometryHumansImmunohistochemistryKaryotypingMesodermPluripotent Stem CellsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNARNACell aggregatesDifferentiationMicrocarriersPluripotent stem cellsScalable cultureSpinner flasksXeno-free culture

Identifiers

PMID26659793
PMCPMC5642038
OpenAlexW2410182695

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.