ArticlePloS one2015
Investigation of Reference Genes in Vibrio parahaemolyticus for Gene Expression Analysis Using Quantitative RT-PCR.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Tetrathionate sensing enablesGut microbes · 2026Article
- Transcriptomic dissection of the ToxR regulon in Vibrio parahaemolyticus at low cell density.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Strain-level heterogeneity in Vibrio parahaemolyticus limits the predictive value of baseline planktonic gene expression for surface-associated phenotypes.Scientific reports · 2026Article
- T3SS1 accessory protein geneFrontiers in cellular and infection microbiology · 2026Article
- AphA-dependent c-di-GMP production inJournal of bacteriology · 2025Article
- Evaluation of Suitable Reference Gene During the Development of Paired or Unpaired FemalePathogens (Basel, Switzerland) · 2025Article
- Quantitative Evaluation of Endogenous Reference Genes for RT-qPCR and ddPCR Gene Expression Under Polyextreme Conditions Using Anaerobic Halophilic AlkalithermophileMicroorganisms · 2025Article
- Article
- Environmentally Relevant Concentrations of Tetracycline Promote Horizontal Transfer of Antimicrobial Resistance Genes via Plasmid-Mediated Conjugation.Foods (Basel, Switzerland) · 2024Article
- Quantitative evaluation of endogenous reference genes for ddPCR under salt stress using a moderate halophile.Extremophiles : life under extreme conditions · 2023Article
- Staying below the Radar: Unraveling a New Family of Ubiquitous "Cryptic" Non-Tailed Temperate Vibriophages and Implications for Their Bacterial Hosts.International journal of molecular sciences · 2023Article
- Haloalkaline Lipase from Bacillus flexus PU2 Efficiently Inhibits Biofilm Formation of Aquatic Pathogen Vibrio parahaemolyticus.Probiotics and antimicrobial proteins · 2022Article
- Comparative Survival and the Cold-Induced Gene Expression of Pathogenic and NonpathogenicInternational journal of environmental research and public health · 2020Article
- Stress Response ofFrontiers in microbiology · 2020Article
- QseC Inhibition as a Novel Antivirulence Strategy for the Prevention of Acute Hepatopancreatic Necrosis Disease (AHPND)-CausingFrontiers in cellular and infection microbiology · 2020Article
- Analysis of theFrontiers in cellular and infection microbiology · 2020Article
- Cold shock induces chromosomalAntimicrobial agents and chemotherapy · 2019Article
- Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions.Extremophiles : life under extreme conditions · 2019Article
- Conservation of Small Regulatory RNAs inInternational journal of molecular sciences · 2019Article
- Potential Survival and Pathogenesis of a Novel Strain,Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio parahaemolyticus is a significant human pathogen capable of causing foodborne gastroenteritis associated with the consumption of contaminated raw or undercooked seafood. Quantitative RT-PCR (qRT-PCR) is a useful tool for studying gene expression in V. parahaemolyticus to characterize its virulence factors and understand the effect of environmental conditions on its pathogenicity. However, there is not a stable gene in V. parahaemolyticus that has been identified for use as a reference gene for qRT-PCR. This study evaluated the stability of 6 reference genes (16S rRNA, recA, rpoS, pvsA, pvuA, and gapdh) in 5 V. parahaemolyticus strains (O3:K6-clinical strain-tdh+, ATCC33846-tdh+, ATCC33847-tdh+, ATCC17802-trh+, and F13-environmental strain-tdh+) cultured at 4 different temperatures (15, 25, 37 and 42°C). Stability values were calculated using GeNorm, NormFinder, BestKeeper, and Delta CT algorithms. The results indicated that recA was the most stably expressed gene in the V. parahaemolyticus strains cultured at different temperatures. This study examined multiple V. parahaemolyticus strains and growth temperatures, hence the finding provided stronger evidence that recA can be used as a reference gene for gene expression studies in V. parahaemolyticus.
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