ArticleJournal of clinical microbiology2016
Evaluation of the Bruker MALDI Biotyper for Identification of Fastidious Gram-Negative Rods.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
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- Feasibility of anaerobic co-digestion for biogas production from recycled paper industry sludge: optimization of mixing ratios and application in two-stage CSTR system design.Water science and technology : a journal of the International Association on Water Pollution Research · 2025Article
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- Identification methods as a factor affecting the performance of clinical microbiology laboratories participating in an external quality assessment program: a cross-sectional, retrospective analysis.Journal of medical microbiology · 2024Article
- MALDI glycotyping of O-antigens from a single colony of gram-negative bacteria.Scientific reports · 2024Article
- Association between Moraxella keratitis and advanced glycation end products.Scientific reports · 2024Article
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- Comparison of MALDI-TOF mass spectrometry and 16S rDNA sequencing for identification of environmental bacteria: a case study of cave mussel-associated culturable microorganisms.Environmental science and pollution research international · 2024Article
- Engineered reporter phages for detection of Escherichia coli, Enterococcus, and Klebsiella in urine.Nature communications · 2023Article
- Performance of Two Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci.Infection and drug resistance · 2023Article
- Isolation, identification, and significance of salivaryFrontiers in cellular and infection microbiology · 2023Article
- Limitation ofJournal of clinical microbiology · 2022Article
- Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites.BMC microbiology · 2022Article
- Microbiological Quality of Milk Donated to the Regional Human Milk Bank in Warsaw in the First Four Years of Activity.Healthcare (Basel, Switzerland) · 2022Article
- Distribution of CRISPR Types in Fluoroquinolone-ResistantPathogens (Basel, Switzerland) · 2021Article
- Draft Genome Sequences ofMicrobiology resource announcements · 2021Article
- Moraxella keratitis: epidemiology and outcomes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has entered clinical laboratories, facilitating identification of bacteria. Here, we evaluated the MALDI Biotyper (Bruker Daltonics) for the identification of fastidious Gram-negative rods (GNR). Three sample preparation methods, direct colony transfer, direct transfer plus on-target formic acid preparation, and ethanol-formic acid extraction, were analyzed for 151 clinical isolates. Direct colony transfer applied with the manufacturer's interpretation criteria resulted in overall species and genus identification rates of 43.0% and 32.5%, respectively; 23.2% of the isolates were not identified, and two misidentifications (1.3%) were observed. The species identification rates increased to 46.4% and 53.7% for direct transfer plus formic acid preparation and ethanol-formic acid extraction, respectively. In addition, we evaluated score value cutoff alterations. The identification rates hardly increased by reducing the genus cutoff, while reducing the 2.0 species cutoff to 1.9 and to 1.8 increased the identification rates to up to 66.2% without increasing the rate of misidentifications. This study shows that fastidious GNR can reliably be identified using the MALDI Biotyper. However, the identification rates do not reach those of nonfastidious GNR such as the Enterobacteriaceae. In addition, two approaches optimizing the identification of fastidious GNR by the MALDI Biotyper were demonstrated: formic acid-based on-target sample treatment and reductions in cutoff scores to increase the species identification rates.
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