ArticlePloS one2015
The Role of Glutamate Dehydrogenase (GDH) Testing Assay in the Diagnosis of Clostridium difficile Infections: A High Sensitive Screening Test and an Essential Step in the Proposed Laboratory Diagnosis Workflow for Developing Countries like China.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Laboratory Diagnostic Methods forAnnals of laboratory medicine · 2021Pooled it
- Can Gut Microbiota Analysis RevealMicroorganisms · 2024Article
- Culture-independent detection of low-abundantApplied and environmental microbiology · 2024Article
- Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR.Microbiology spectrum · 2023Article
- Rapid-format recombinant antibody-based methods for the diagnosis ofFrontiers in microbiology · 2022Review
- Isolation, molecular typing and antimicrobial resistance ofFrontiers in veterinary science · 2022Article
- Evaluation of an immunochromatographic test for the detection of glutamate dehydrogenase for the diagnosis of Clostridioides (Clostridium) difficile infection in dogs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Frequency of toxin genes and antibiotic resistance pattern ofGastroenterology and hepatology from bed to bench · 2021Article
- Evaluation of glutamate dehydrogenase (GDH) and toxin A/B rapid tests for Clostridioides (prev. Clostridium) difficile diagnosis in a university hospital in Minas Gerais, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Antimicrobial Susceptibilities of Clostridium difficile Isolates from 12 Asia-Pacific Countries in 2014 and 2015.Antimicrobial agents and chemotherapy · 2020Article
- Article
- Comparative Study ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Clostridium difficile-associated Diarrhea in Developing Countries: A Systematic Review and Meta-Analysis.Infectious diseases and therapy · 2019Article
- Molecular-based diagnosis of Clostridium difficile infection is associated with reduced mortality.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Molecular epidemiology ofInfection and drug resistance · 2018Article
- Evaluation of Copan FecalSwab as Specimen Type for Use in Xpert C. difficile Assay.Journal of clinical microbiology · 2017Article
- Current knowledge on the laboratory diagnosis ofWorld journal of gastroenterology · 2017Review
- The Daniel K. Inouye College of Pharmacy Scripts: Updates onHawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health · 2017Article
- Prevalence and molecular epidemiology ofNew microbes and new infections · 2017Article
- [Clostridium difficile infection : What is currently available for treatment?]Der Internist · 2016Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incidence and severity of Clostridium difficile infection (CDI) in North America and Europe has increased significantly since the 2000s. However, CDI is not widely recognized in China and other developing countries due to limited laboratory diagnostic capacity and low awareness. Most published studies on laboratory workflows for CDI diagnosis are from developed countries, and thus may not be suitable for most developing countries. Therefore, an alternative strategy for developing countries is needed. In this study, we evaluated the performance of the Glutamate Dehydrogenase (GDH) test and its associated workflow on 416 fecal specimens from suspected CDI cases. The assay exhibited excellent sensitivity (100.0%) and specificity (92.8%), compared to culture based method, and thus could be a good screening marker for C. difficile but not for indication of toxin production. The VIDAS CDAB assay, which can detect toxin A/B directly from fecal specimens, showed good specificity (99.7%) and positive predictive value (97.2%), but low sensitivity (45.0%) and negative predictive value (88.3%), compared with PCR-based toxin gene detection. Therefore, we propose a practical and efficient GDH test based workflow strategy for the laboratory diagnosis of CDI in developing countries like China. By applying this new workflow, the CDI laboratory diagnosis rate was notably improved in our center, yet the increasing cost was kept at a minimum level. Furthermore, to gain some insights into the genetic population structure of C. difficile isolates from our hospital, we performed MLST and PCR toxin gene typing.
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