ArticlePLoS genetics2015
The Drosophila Zinc Finger Transcription Factor Ouija Board Controls Ecdysteroid Biosynthesis through Specific Regulation of spookier.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- 20-Hydroxyecdysone primary response gene LmBlimp-1 controls locust molt and survival by regulating chitin metabolism.Pest management science · 2026Article
- Genetic and Molecular Regulation of Ecdysone Biosynthesis During Insect Metamorphosis.Insects · 2026Review
- AZoological research · 2026Article
- Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins inScience advances · 2026Article
- Single-cell transcriptomics of theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- ZAD mediates chromatin binding and insulator activity of Drosophila Pita and can be replaced with the human ZFP276 ZAD-like domain.Epigenetics & chromatin · 2025Article
- Nutrient status alters developmental fates via a switch in mitochondrial homeodynamics.Nature communications · 2025Article
- Early attainment of 20-hydroxyecdysone threshold shapes mosquito sexual dimorphism in developmental timing.Nature communications · 2025Article
- Developmental analysis of Spalt function in the Drosophila prothoracic gland.Development (Cambridge, England) · 2024Article
- Study of the Association of Ouib and Nom with Heterochromatin in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2023Article
- Twenty-seven ZAD-ZNF genes of Drosophila melanogaster are orthologous to the embryo polarity determining mosquito gene cucoid.PloS one · 2023Article
- Article
- Transcriptional Regulators of Ecdysteroid Biosynthetic Enzymes and Their Roles in Insect Development.Frontiers in physiology · 2022Review
- Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development.BMC biology · 2021Article
- Structural basis of diversity and homodimerization specificity of zinc-finger-associated domains in Drosophila.Nucleic acids research · 2021Article
- Innovation of heterochromatin functions drives rapid evolution of essential ZAD-ZNF genes ineLife · 2020Article
- Regulation of Body Size and Growth Control.Genetics · 2020Review
- Snail synchronizes endocycling in a TOR-dependent manner to coordinate entry and escape from endoreplication pausing during the Drosophila critical weight checkpoint.PLoS biology · 2020Article
- Poly(A) Binding Protein Is Required for Nuclear Localization of the Ecdysteroidogenic Transcription Factor Molting Defective in the Prothoracic Gland ofFrontiers in genetics · 2020Article
- AG3 (Bethesda, Md.) · 2018Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Steroid hormones are crucial for many biological events in multicellular organisms. In insects, the principal steroid hormones are ecdysteroids, which play essential roles in regulating molting and metamorphosis. During larval and pupal development, ecdysteroids are synthesized in the prothoracic gland (PG) from dietary cholesterol via a series of hydroxylation and oxidation steps. The expression of all but one of the known ecdysteroid biosynthetic enzymes is restricted to the PG, but the transcriptional regulatory networks responsible for generating such exquisite tissue-specific regulation is only beginning to be elucidated. Here, we report identification and characterization of the C2H2-type zinc finger transcription factor Ouija board (Ouib) necessary for ecdysteroid production in the PG in the fruit fly Drosophila melanogaster. Expression of ouib is predominantly limited to the PG, and genetic null mutants of ouib result in larval developmental arrest that can be rescued by administrating an active ecdysteroid. Interestingly, ouib mutant animals exhibit a strong reduction in the expression of one ecdysteroid biosynthetic enzyme, spookier. Using a cell culture-based luciferase reporter assay, Ouib protein stimulates transcription of spok by binding to a specific ~15 bp response element in the spok PG enhancer element. Most remarkable, the developmental arrest phenotype of ouib mutants is rescued by over-expression of a functionally-equivalent paralog of spookier. These observations imply that the main biological function of Ouib is to specifically regulate spookier transcription during Drosophila development.
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