ArticleScientific reports2015
Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria.Biofilm · 2025Article
- Targeted volume imaging reveals early vascular interactions of Lyme disease pathogen in skin.Nature communications · 2025Article
- Navigating infection by pathogenic spirochetes: The host-bacteria interface at the atomic level.Protein science : a publication of the Protein Society · 2025Review
- Correlative light and electron microscopy imaging of proteinaceous deposits in cell cultures and brain tissues.Acta neuropathologica communications · 2025Article
- Bright New Resources for Syphilis Research: Genetically Encoded Fluorescent Tags for Treponema pallidum and Sf1Ep Cells.Molecular microbiology · 2024Article
- Traceable localization enables accurate integration of quantum emitters and photonic structures with high yield.Optica quantum · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures.Scientific reports · 2022Article
- Distinctive Evasion Mechanisms to Allow Persistence ofFrontiers in microbiology · 2021Article
- Novel targets and strategies to combat borreliosis.Applied microbiology and biotechnology · 2020Review
- Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Cryo-laser scanning confocal microscopy of diffusible plant compounds.Plant methods · 2018Article
- Pleomorphism and Viability of the Lyme Disease PathogenFrontiers in microbiology · 2017Article
- Towards correlative super-resolution fluorescence and electron cryo-microscopy.Biology of the cell · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution. We established a novel correlative cryo-fluorescence microscopy and cryo-scanning electron microscopy workflow, which enables imaging of the studied object of interest very close to its natural state, devoid of artifacts caused for instance by slow chemical fixation. This system was tested by investigating the interaction of the zoonotic bacterium Borrelia burgdorferi with two mammalian cell lines of neural origin in order to broaden our knowledge about the cell-association mechanisms that precedes the entry of the bacteria into the cell. This method appears to be an unprecedentedly fast (<3 hours), straightforward, and reliable solution to study the finer details of pathogen-host cell interactions and provides important insights into the complex and dynamic relationship between a pathogen and a host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.