ArticleInvestigative ophthalmology & visual science2015
High-Magnitude and/or High-Frequency Mechanical Strain Promotes Peripapillary Scleral Myofibroblast Differentiation.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Revisiting the sclera as a target for glaucoma therapy.Eye (London, England) · 2026Review
- Analysis of 2-dimensional regional differences in the peripapillary scleral fibroblast cytoskeleton of normotensive and hypertensive mouse eyes.Scientific reports · 2025Article
- IOP and glaucoma damage: The essential role of optic nerve head and retinal mechanosensors.Progress in retinal and eye research · 2024Review
- CXC- receptor 2 promotes extracellular matrix production and attenuates migration in peripapillary human scleral fibroblasts under mechanical strain.Journal of cellular and molecular medicine · 2022Article
- Remodeling of the Lamina Cribrosa: Mechanisms and Potential Therapeutic Approaches for Glaucoma.International journal of molecular sciences · 2022Review
- Strain by virtual extensometers and video-imaging optical coherence tomography as a repeatable metric for IOP-Induced optic nerve head deformations.Experimental eye research · 2021Article
- Racial Differences in the Rate of Change in Anterior Lamina Cribrosa Surface Depth in the African Descent and Glaucoma Evaluation Study.Investigative ophthalmology & visual science · 2021Article
- Review
- Regional Differences and Physiologic Behaviors in Peripapillary Scleral Fibroblasts.Investigative ophthalmology & visual science · 2021Article
- Mechanical Strain Regulates Myofibroblast Differentiation of Human Scleral Fibroblasts by YAP.Frontiers in physiology · 2021Article
- Comparison of extraocular and intraocular pressure transducers for measurement of transient intraocular pressure fluctuations using continuous wireless telemetry.Scientific reports · 2020Article
- Connective Tissue Remodeling in Myopia and its Potential Role in Increasing Risk of Glaucoma.Current opinion in biomedical engineering · 2020Article
- Dasatinib inhibits peripapillary scleral myofibroblast differentiation.Experimental eye research · 2020Article
- Diurnal Cycle of Translaminar Pressure in Nonhuman Primates Quantified With Continuous Wireless Telemetry.Investigative ophthalmology & visual science · 2020Article
- The Dynamic Scleral Extracellular Matrix Alterations in Chronic Ocular Hypertension Model of Rats.Frontiers in physiology · 2020Article
- Scleral structure and biomechanics.Progress in retinal and eye research · 2020Review
- Cyclic Pattern of Intraocular Pressure (IOP) and Transient IOP Fluctuations in Nonhuman Primates Measured with Continuous Wireless Telemetry.Current eye research · 2019Article
- Transient Intraocular Pressure Fluctuations: Source, Magnitude, Frequency, and Associated Mechanical Energy.Investigative ophthalmology & visual science · 2019Article
- Rho-Kinase Inhibition Reduces Myofibroblast Differentiation and Proliferation of Scleral Fibroblasts Induced by Transforming Growth Factor β and Experimental Glaucoma.Translational vision science & technology · 2018Article
- Lamina cribrosa in glaucoma.Current opinion in ophthalmology · 2017Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
Abstract
purposeTo determine the effects of altered mechanical strain on human peripapillary scleral (ppSc) fibroblast-to-myofibroblast differentiation.
methodsEight human ppSc fibroblast cultures were isolated from three paired eyes and two unilateral eyes of five donors using an explant approach. Human ppSc fibroblast isolates were subjected to 1% and 4% cyclic strain at 0.05 to 5 Hz for 24 hours. Levels of α smooth muscle actin (αSMA) mRNA and protein were determined by real-time PCR and immunoblot. Incorporation of αSMA into actin stress fibers was evaluated by confocal immunofluorescent microscopy. Myofibroblast contractility was measured by fibroblast-populated three-dimensional collagen gel contraction assay and phosphorylation of myosin light chain (MLC20).
resultsHuman ppSc fibroblasts contained 6% to 47% fully differentiated myofibroblasts before strain application; 4% cyclic strain increased αSMA mRNA and protein expression in ppSc fibroblasts compared with 1% strain applied at 5 Hz, but not at lower frequencies. Seven of eight ppSc fibroblast isolates responded to high-magnitude and high-frequency strain with increased cellular contractility and increased MLC20 phosphorylation. In addition, increasing strain frequency promoted αSMA expression in ppSc fibroblasts under both 1% and 4% strain conditions.
conclusionsHigh-magnitude and/or high-frequency mechanical strain promotes differentiation of human ppSc fibroblasts into contractile myofibroblasts, a fibroblast phenotypic change known to be key to tissue injury-repair responses. These findings suggest that the cellular constituent of ppSc may play an important role in the regulation of optic nerve head biomechanics in response to injurious IOP fluctuations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.