ArticleScientific reports2015
Biofortification of oilseed Brassica juncea with the anti-cancer compound glucoraphanin by suppressing GSL-ALK gene family.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 86 citations in OpenAlex.
- Glucosinolates inInternational journal of molecular sciences · 2026Review
- QTL and candidate gene analysis unveil genetic control of floret aliphatic glucosinolate side-chain modification inHorticulture research · 2025Article
- A Comprehensive Review of Germination Impact on Moringa Seeds and Sprouts: Physiological and Biochemical Changes, Bioactive Compounds, Health Benefits, and Food Applications.Comprehensive reviews in food science and food safety · 2025Review
- High-level production of health-beneficial glucoraphanin by multiplex editing of AOP2 gene family in mustard.Plant biotechnology journal · 2025Article
- Gap-free genome assembly and pan-genome of Brassica juncea provide insights into seed quality improvement and environmental adaptation.Plant communications · 2025Article
- Article
- Comprehensive advances in phytochemical components, bioactive functionality, and processing applications of mustard (Frontiers in nutrition · 2025Review
- Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways.Plant communications · 2023Review
- Innovative applications and therapeutic potential of oilseeds and their by-products: An eco-friendly and sustainable approach.Food science & nutrition · 2023Review
- Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.Genes · 2023Article
- Genome-Wide Association Study of Glucosinolate Metabolites (mGWAS) inPlants (Basel, Switzerland) · 2023Article
- Identification andFrontiers in plant science · 2023Article
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- Variation in the Main Health-Promoting Compounds and Antioxidant Activity of Different Edible Parts of Purple Flowering Stalks (Plants (Basel, Switzerland) · 2022Article
- Glucoraphanin Increases Intracellular Hydrogen Sulfide (HNutrients · 2022Article
- Extra-large G-proteins influence plant response to Sclerotinia sclerotiorum by regulating glucosinolate metabolism in Brassica juncea.Molecular plant pathology · 2021Article
- Improvement of glucosinolates by metabolic engineering inaBIOTECH · 2021Review
- Effects of Glucosinolate-Derived Isothiocyanates on Fungi: A Comprehensive Review on Direct Effects, Mechanisms, Structure-Activity Relationship Data and Possible Agricultural Applications.Journal of fungi (Basel, Switzerland) · 2021Review
- Challenges and Opportunities of Light-Emitting Diode (LED) as Key to Modulate Antioxidant Compounds in Plants. A Review.Antioxidants (Basel, Switzerland) · 2020Review
- Effects of Supplementary Blue and UV-A LED Lights on Morphology and Phytochemicals ofMolecules (Basel, Switzerland) · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucosinolates are amino acids derived secondary metabolites, invariably present in Brassicales, which have huge health and agricultural benefits. Sulphoraphane, the breakdown product of glucosinolate glucoraphanin is known to posses anti-cancer properties. AOP (2-oxoglutarate-dependent dioxygenases) or GSL-ALK enzyme catalyzes the conversion of desirable glucoraphanin to deleterious gluconapin and progoitrin, which are present in very high amounts in most of the cultivable Brassica species including Brassica juncea. In this study we showed that B. juncea encodes four functional homologs of GSL-ALK gene and constitutive silencing of GSL-ALK homologs resulted in accumulation of glucoraphanin up to 43.11 μmoles g(-1) DW in the seeds with a concomitant reduction in the anti-nutritional glucosinolates. Glucoraphanin content was found remarkably high in leaves as well as sprouts of the transgenic lines. Transcript quantification of high glucoraphanin lines confirmed significant down-regulation of GSL-ALK homologs. Growth and other seed quality parameters of the transgenic lines did not show drastic difference, compared to the untransformed control. High glucoraphanin lines also showed higher resistance towards stem rot pathogen Sclerotinia sclerotiorum. Our results suggest that metabolic engineering of GSL-ALK has huge potential for enriching glucoraphanin content, and improve the oil quality and vegetable value of Brassica crops.
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