ArticleScientific reports2015
MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Exon skipping peptide-conjugated morpholinos downregulate dynamin 2 to rescue centronuclear myopathy.Brain : a journal of neurology · 2025Article
- The Role of the Extracellular Matrix in Inducing Cardiac Cell Regeneration and Differentiation.Cells · 2025Review
- The miR-17-92 cluster in cardiac health and disease.Birth defects research · 2024Review
- Post-Transcriptional Regulation of Molecular Determinants during Cardiogenesis.International journal of molecular sciences · 2022Review
- From Stripes to a Beating Heart: Early Cardiac Development in Zebrafish.Journal of cardiovascular development and disease · 2021Review
- Zebrafish Models for Human Skeletal Disorders.Frontiers in genetics · 2021Review
- Novel mutations of TCTN3/LTBP2 with cellular function changes in congenital heart disease associated with polydactyly.Journal of cellular and molecular medicine · 2020Article
- miR-182-5p is an evolutionarily conserved Tbx5 effector that impacts cardiac development and electrical activity in zebrafish.Cellular and molecular life sciences : CMLS · 2020Article
- Dysregulated micro-RNAs and long noncoding RNAs in cardiac development and pediatric heart failure.American journal of physiology. Heart and circulatory physiology · 2020Review
- Epigenetic Regulation of Organ Regeneration in Zebrafish.Journal of cardiovascular development and disease · 2018Review
- Post-transcriptional Modulation of Sphingosine-1-Phosphate Receptor 1 by miR-19a Affects Cardiovascular Development in Zebrafish.Frontiers in cell and developmental biology · 2018Article
- MicroRNAs: pleiotropic players in congenital heart disease and regeneration.Journal of thoracic disease · 2017Review
- miR-19b Regulates Ventricular Action Potential Duration in Zebrafish.Scientific reports · 2016Article
- Dicer ablation in osteoblasts by Runx2 driven cre-loxP recombination affects bone integrity, but not glucocorticoid-induced suppression of bone formation.Scientific reports · 2016Article
- Discovering miRNA Regulatory Networks in Holt-Oram Syndrome Using a Zebrafish Model.Frontiers in bioengineering and biotechnology · 2016Article
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Authors and funding
13 authors.
Funding
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Abstract
Holt-Oram Syndrome (HOS) is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene, a transcription factor capable of regulating hundreds of cardiac-specific genes through complex transcriptional networks. Here we show that, in zebrafish, modulation of a single miRNA is sufficient to rescue the morphogenetic defects generated by HOS. The analysis of miRNA-seq profiling revealed a decreased expression of miR-19a in Tbx5-depleted zebrafish embryos compared to the wild type. We revealed that the transcription of the miR-17-92 cluster, which harbors miR-19a, is induced by Tbx5 and that a defined dosage of miR-19a is essential for the correct development of the heart. Importantly, we highlighted that miR-19a replacement is able to rescue cardiac and pectoral fin defects and to increase the viability of HOS zebrafish embryos. We further observed that miR-19a replacement shifts the global gene expression profile of HOS-like zebrafish embryos towards the wild type condition, confirming the ability of miR-19a to rescue the Tbx5 phenotype. In conclusion our data demonstrate the importance of Tbx5/miR-19a regulatory circuit in heart development and provide a proof of principle that morphogenetic defects associated with HOS can be rescued by transient miRNA modulation.
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