Evidence map›Paper›PMID 26656852›Full record

ArticleScientific reports2015

A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays.

Alessio Polacchini, Giuliana Metelli, Ruggiero Francavilla, Gabriele Baj, Marina Florean, Luca Giovanni Mascaretti, Enrico Tongiorgi

2 registry-linked trialsOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 141 papers, 16 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
141citing papers in PubMed, 16 pooled it
5.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04185298 naterminatednot on this mapstarted 2019, after this paper: background citation

Mobile Technology-Based Simultaneous Aerobic Exercise and Memory Training Intervention for Older Adults With Mild Cognitive Impairment

TypeinterventionalSponsorJennifer BramenRan2019 to 2023Enrolled12ConditionsAmnestic Mild Cognitive ImpairmentArmsmSIM intervention plus activity monitoring, Activity monitoring
NCT07316387 enrolling by invitationnot on this mapstarted 2024, after this paper: background citation

Neurobehavioral Development in Toddlers and Preschoolers in Relation to Prenatal Exposure of Mild Analgesics

TypeobservationalSponsorRigshospitalet, DenmarkRan2024 to 2028Enrolled685ConditionsGonad Regulating Hormone Adverse Reaction, Analgesic Adverse Reaction, Developmental Disability, Adhd
3 · Its place in the literature

Who cites it

141 citing papers in PubMed, 16 syntheses or guidelines pooled it, 250 citations in OpenAlex.

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  11. The Effects of Exercise on BDNF Levels in Adolescents: A Systematic Review with Meta-Analysis.International journal of environmental research and public health · 2020
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  16. Meta-Analysis of BDNF Levels in Autism.Cellular and molecular neurobiology · 2017
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  19. A multidomain trial for cognition in women with cardiovascular disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025
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81 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alessio PolacchiniDepartment of Life Sciences, University of Trieste, 34127 Trieste, Italy.
Giuliana MetelliDepartment of Life Sciences, University of Trieste, 34127 Trieste, Italy.
Ruggiero FrancavillaDepartment of Life Sciences, University of Trieste, 34127 Trieste, Italy.
Gabriele BajDepartment of Life Sciences, University of Trieste, 34127 Trieste, Italy.
Marina FloreanDepartment of Transfusion Medicine, Trieste University Hospital, 34142 Trieste, Italy.
Luca Giovanni MascarettiDepartment of Transfusion Medicine, Trieste University Hospital, 34142 Trieste, Italy.
Enrico TongiorgiDepartment of Life Sciences, University of Trieste, 34127 Trieste, Italy.
University of Trieste · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brain-Derived Neurotrophic Factor (BDNF) has attracted increasing interest as potential biomarker to support the diagnosis or monitor the efficacy of therapies in brain disorders. Circulating BDNF can be measured in serum, plasma or whole blood. However, the use of BDNF as biomarker is limited by the poor reproducibility of results, likely due to the variety of methods used for sample collection and BDNF analysis. To overcome these limitations, using sera from 40 healthy adults, we compared the performance of five ELISA kits (Aviscera-Bioscience, Biosensis, Millipore-ChemiKine(TM), Promega-Emax(®), R&D-System-Quantikine(®)) and one multiplexing assay (Millipore-Milliplex(®)). All kits showed 100% sample recovery and comparable range. However, they exhibited very different inter-assay variations from 5% to 20%. Inter-assay variations were higher than those declared by the manufacturers with only one exception which also had the best overall performance. Dot-blot analysis revealed that two kits selectively recognize mature BDNF, while the others reacted with both pro-BDNF and mature BDNF. In conclusion, we identified two assays to obtain reliable measurements of human serum BDNF, suitable for future clinical applications.

Indexed as

BiomarkersAdultAgedBrain-Derived Neurotrophic FactorEnzyme-Linked Immunosorbent AssayFemaleHealthy VolunteersHumansMaleMiddle AgedReagent Kits, DiagnosticReproducibility of ResultsSensitivity and SpecificityBiomarkersBrain-Derived Neurotrophic FactorReagent Kits, Diagnostic

Identifiers

PMID26656852
PMCPMC4675070
OpenAlexW2190736259

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.