ArticlePloS one2015
Comparative Transcriptome Analysis of Recessive Male Sterility (RGMS) in Sterile and Fertile Brassica napus Lines.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
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- Comparative transcriptome analysis provides insight into the important pathways and key genes related to the pollen abortion in the thermo-sensitive genic male sterile line 373S in Brassica napus L.Functional & integrative genomics · 2022Article
- Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil theInternational journal of molecular sciences · 2022Article
- Transcriptome and Small RNA Sequencing Reveal the Mechanisms Regulating Harvest Index inFrontiers in plant science · 2022Article
- The characterization and candidate gene isolation for a novel male-sterile mutant ms40 in maize.Plant cell reports · 2021Article
- Transcriptome Profiling Reveals Molecular Changes during Flower Development between Male Sterile and Fertile Chinese Cabbage (Life (Basel, Switzerland) · 2021Article
- RNA Sequencing Reveals Rice Genes Involved in Male Reproductive Development under Temperature Alteration.Plants (Basel, Switzerland) · 2021Article
- Genome-wide identification and comparative analysis of diacylglycerol kinase (DGK) gene family and their expression profiling in Brassica napus under abiotic stress.BMC plant biology · 2020Article
- Integrative analysis of transcriptomic and proteomic changes related to male sterility inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
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- Analysis of differentially expressed genes and pathways associated with male sterility lines in watermelon via bulked segregant RNA-seq.3 Biotech · 2020Article
- Investigation of the genes associated with a male sterility mutant (msm) in Chinese cabbage (Brassica campestris ssp. pekinensis) using RNA-Seq.Molecular genetics and genomics : MGG · 2020Article
- Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding.Plant reproduction · 2019Review
- Transcriptomic analysis reveals the mechanism of thermosensitive genic male sterility (TGMS) of Brassica napus under the high temperature inducement.BMC genomics · 2019Article
- Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.).BMC plant biology · 2019Article
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- Article
- Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems inInternational journal of molecular sciences · 2018Article
- Association Mapping Analysis of Fatty Acid Content in Different Ecotypic Rapeseed Using mrMLM.Frontiers in plant science · 2018Article
- De novo transcriptome reconstruction of a thermo-sensitive male sterility mutant in rapeseed (Applications in plant sciences · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recessive genetic male sterility (RGMS) system plays a key role in the production of hybrid varieties in self-pollinating B. napus plants, and prevents negative cytoplasmic effects. However, the complete molecular mechanism of the male sterility during male-gametogenesis in RGMS remains to be determined. To identify transcriptomic changes that occur during the transition to male sterility in RGMS, we examined the male sterile line WSLA and male fertile line WSLB, which are near-isogenic lines (NILs) differing only in the fertility trait. We evaluated the phenotypic features and sterility stage using anatomical analysis. Comparative RNA sequencing analysis revealed that 3,199 genes were differentially expressed between WSLA and WSLB. Many of these genes are mainly involved in biological processes related to flowering, including pollen tube development and growth, pollen wall assembly and modification, and pollen exine formation and pollination. The transcript profiles of 93 genes associated with pollen wall and anther development were determined by quantitative RT-PCR in different flower parts, and classified into the following three major clades: (1) up-regulated in WSLA plants; (2) down-regulated in WSLA plants; and 3) down-regulated in buds, but have a higher expression in stigmas of WSLA than in WSLB. A subset of genes associated with sporopollenin accumulation were all up-regulated in WSLA. An excess of sporopollenin results in defective pollen wall formation, which leads to male sterility in WSLA. Some of the genes identified in this study are candidates for future research, as they could provide important insight into the molecular mechanisms underlying RGMS in WSLA.
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