ArticleNucleic acids research2016
RNA-seq reveals post-transcriptional regulation of Drosophila insulin-like peptide dilp8 and the neuropeptide-like precursor Nplp2 by the exoribonuclease Pacman/XRN1.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 22 citations in OpenAlex.
- A mdg4 retrotransposon screen for X-linked female sterile alleles and its relationship with the transcription factor OVO.G3 (Bethesda, Md.) · 2026Article
- miR-184 modulates Ilp8 to control developmental timing during normal growth conditions and in response to developmental perturbations.Development (Cambridge, England) · 2026Article
- AbioRxiv : the preprint server for biology · 2026Article
- TAF1 is required for fetal but not adult hematopoiesis in mice.Developmental cell · 2025Article
- Characterisation of the in-vivo miRNA landscape in Drosophila ribonuclease mutants reveals Pacman-mediated regulation of the highly conserved let-7 cluster during apoptotic processes.Frontiers in genetics · 2024Article
- Genome-wide analyses of XRN1-sensitive targets in osteosarcoma cells identify disease-relevant transcripts containing G-rich motifs.RNA (New York, N.Y.) · 2021Article
- Article
- Systemic signalling and local effectors in developmental stability, body symmetry, and size.Cell stress · 2018Review
- Mbf1 ensures Polycomb silencing by protectingDevelopment (Cambridge, England) · 2018Article
- Neuronal function of the mRNA decapping complex determines survival ofOpen biology · 2017Article
- A novel role for the 3'-5' exoribonuclease Dis3L2 in controlling cell proliferation and tissue growth.RNA biology · 2016Article
- Characterization of Reproductive Dormancy in MaleFrontiers in physiology · 2016Article
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5 authors at 1 institution in 1 country.
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Abstract
Ribonucleases are critically important in many cellular and developmental processes and defects in their expression are associated with human disease. Pacman/XRN1 is a highly conserved cytoplasmic exoribonuclease which degrades RNAs in a 5'-3' direction. In Drosophila, null mutations in pacman result in small imaginal discs, a delay in onset of pupariation and lethality during the early pupal stage. In this paper, we have used RNA-seq in a genome-wide search for mRNAs misregulated in pacman null wing imaginal discs. Only 4.2% of genes are misregulated ±>2-fold in pacman null mutants compared to controls, in line with previous work showing that Pacman has specificity for particular mRNAs. Further analysis of the most upregulated mRNAs showed that Pacman post-transcriptionally regulates the expression of the secreted insulin-like peptide Dilp8. Dilp8 is related to human IGF-1, and has been shown to coordinate tissue growth with developmental timing in Drosophila. The increased expression of Dilp8 is consistent with the developmental delay seen in pacman null mutants. Our analysis, together with our previous results, show that the normal role of this exoribonuclease in imaginal discs is to suppress the expression of transcripts that are crucial in apoptosis and growth control during normal development.
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