ArticleScientific reports2015
Nanoparticle-assisted optical tethering of endosomes reveals the cooperative function of dyneins in retrograde axonal transport.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Enhanced Single-Particle Upconversion Imaging via Energy Migration Boosting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- On The Retrograde Transport of RNA-Loaded Lipid Nanoparticles Designed for Brain Delivery.ACS nanoscience Au · 2025Article
- Nanometer-resolution tracking of single cargo reveals dynein motor mechanisms.Nature chemical biology · 2025Article
- Nanomagnetic Guidance Shapes the Structure-Function Relationship of Developing Cortical Networks.Nano letters · 2024Article
- Neural Tracing Protein-Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins.Chemical & biomedical imaging · 2024Article
- Nanoparticles for Interrogation of Cell Signaling.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Effects of dynein inhibitor on the number of motor proteins transporting synaptic cargos.Biophysical journal · 2021Article
- Neuronal delivery of nanoparticles via nerve fibres in the skin.Scientific reports · 2021Article
- Topical Delivery of Four Neuroprotective Ingredients by Ethosome-Gel: Synergistic Combination for Treatment of Oxaliplatin-Induced Peripheral Neuropathy.International journal of nanomedicine · 2020Article
- Detection of Velocity and Diffusion Coefficient Change Points in Single-Particle Trajectories.Biophysical journal · 2018Article
- Dynamic Clustering of Dyneins on Axonal Endosomes: Evidence from High-Speed Darkfield Imaging.Biophysical journal · 2018Article
- Nanoparticles for super-resolution microscopy and single-molecule tracking.Nature methods · 2018Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Dynein-dependent transport of organelles from the axon terminals to the cell bodies is essential to the survival and function of neurons. However, quantitative knowledge of dyneins on axonal organelles and their collective function during this long-distance transport is lacking because current technologies to do such measurements are not applicable to neurons. Here, we report a new method termed nanoparticle-assisted optical tethering of endosomes (NOTE) that made it possible to study the cooperative mechanics of dyneins on retrograde axonal endosomes in live neurons. In this method, the opposing force from an elastic tether causes the endosomes to gradually stall under load and detach with a recoil velocity proportional to the dynein forces. These recoil velocities reveal that the axonal endosomes, despite their small size, can recruit up to 7 dyneins that function as independent mechanical units stochastically sharing load, which is vital for robust retrograde axonal transport. This study shows that NOTE, which relies on controlled generation of reactive oxygen species, is a viable method to manipulate small cellular cargos that are beyond the reach of current technology.
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Registered trials
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