ArticleScientific reports2015
Microspectrometric insights on the uptake of antibiotics at the single bacterial cell level.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Review
- Click-based determination of accumulation of molecules in Escherichia coli.Nature communications · 2026Article
- Bioluminescence-Based Determination of Cytosolic Accumulation of Antibiotics inACS infectious diseases · 2024Article
- Date (Phoenix dactylifera L.) seed oil is an agro-industrial waste with biopreservative effects and antimicrobial activity.Scientific reports · 2023Article
- Broadband cavity enhanced UV-VIS absorption spectroscopy for picolitre liquid samples.The Analyst · 2023Article
- Factors Influencing the Bioavailability of Organic Molecules to Bacterial Cells-A Mini-Review.Molecules (Basel, Switzerland) · 2022Review
- An LC-MS/MS assay and complementary web-based tool to quantify and predict compound accumulation in E. coli.Nature protocols · 2021Review
- The Whole Is Bigger than the Sum of Its Parts: Drug Transport in the Context of Two Membranes with Active Efflux.Chemical reviews · 2021Article
- Determination of Drug Efflux Pump Efficiency in Drug-Resistant Bacteria Using MALDI-TOF MS.Antibiotics (Basel, Switzerland) · 2020Article
- Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria.Lab on a chip · 2020Article
- Synchrotron multimodal imaging in a whole cell reveals lipid droplet core organization.Journal of synchrotron radiation · 2020Article
- The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux.Communications biology · 2020Article
- Molecular Interactions of Carbapenem Antibiotics with the Multidrug Efflux Transporter AcrB ofInternational journal of molecular sciences · 2020Article
- Permeability barriers of Gram-negative pathogens.Annals of the New York Academy of Sciences · 2020Review
- Review
- Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux.MedChemComm · 2019Article
- Reversing resistance to counter antimicrobial resistance in the World Health Organisation's critical priority of most dangerous pathogens.Bioscience reports · 2019Review
- Mechanistic aspects of maltotriose-conjugate translocation to the Gram-negative bacteria cytoplasm.Life science alliance · 2019Article
- Inhibition of Salmonella Typhimurium adhesion, invasion, and intracellular survival via treatment with methyl gallate alone and in combination with marbofloxacin.Veterinary research · 2018Article
- Spectrofluorimetric quantification of antibiotic drug concentration in bacterial cells for the characterization of translocation across bacterial membranes.Nature protocols · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial multidrug resistance is a significant health issue. A key challenge, particularly in Gram-negative antibacterial research, is to better understand membrane permeation of antibiotics in clinically relevant bacterial pathogens. Passing through the membrane barrier to reach the required concentration inside the bacterium is a pivotal step for most antibacterials. Spectrometric methodology has been developed to detect drugs inside bacteria and recent studies have focused on bacterial cell imaging. Ultimately, we seek to use this method to identify pharmacophoric groups which improve penetration, and therefore accumulation, of small-molecule antibiotics inside bacteria. We developed a method to quantify the time scale of antibiotic accumulation in living bacterial cells. Tunable ultraviolet excitation provided by DISCO beamline (synchrotron Soleil) combined with microscopy allows spectroscopic analysis of the antibiotic signal in individual bacterial cells. Robust controls and measurement of the crosstalk between fluorescence channels can provide real time quantification of drug. This technique represents a new method to assay drug translocation inside the cell and therefore incorporate rational drug design to impact antibiotic uptake.
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