ArticleApplied and environmental microbiology2016
Alginate Biosynthesis Factories in Pseudomonas fluorescens: Localization and Correlation with Alginate Production Level.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Alginate-based biomaterials in orthopedics: What are the prospects for bacterial alginate?World journal of orthopedics · 2025Review
- Alginate-like exopolysaccharides extracted from different waste sludges exhibit varying physicochemical and material properties.Frontiers in microbiology · 2024Article
- Highly diverse flavobacterial phages isolated from North Sea spring blooms.The ISME journal · 2022Article
- Article
- Competitive Biosynthesis of Bacterial Alginate UsingPolymers · 2021Article
- Genome Mining and Evaluation of the Biocontrol Potential ofInternational journal of molecular sciences · 2020Article
- Genomic Characterisation of Mushroom Pathogenic Pseudomonads and Their Interaction with Bacteriophages.Viruses · 2020Article
- Ultrasound-mediated therapies for the treatment of biofilms in chronic wounds: a review of present knowledge.Microbial biotechnology · 2020Review
- Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis inFrontiers in bioengineering and biotechnology · 2019Article
- The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.Microbial biotechnology · 2017Review
- Activation Mechanism and Cellular Localization of Membrane-Anchored Alginate Polymerase in Pseudomonas aeruginosa.Applied and environmental microbiology · 2017Article
- Identification of a New Phosphatase Enzyme Potentially Involved in the Sugar Phosphate Stress Response in Pseudomonas fluorescens.Applied and environmental microbiology · 2017Article
- Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library.BMC genomics · 2017Article
- Technological Microbiology: Development and Applications.Frontiers in microbiology · 2017Review
- Biological function of a polysaccharide degrading enzyme in the periplasm.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detected by immunogold labeling and transmission electron microscopy. Localization of the AlgE protein was found to depend on the presence of other proteins in the multiprotein complex. No correlation was found between the number of alginate factories and the alginate production level, nor were the numbers of these factories affected in an algC mutant that is unable to produce the precursor needed for alginate biosynthesis. Precursor availability and growth phase thus seem to be the main determinants for the alginate production rate in our strain. Clustering analysis demonstrated that the alginate multiprotein complexes were not distributed randomly over the entire outer cell membrane surface.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.