ArticleProceedings of the National Academy of Sciences of the United States of America2015
Structural basis for specificity and promiscuity in a carrier protein/enzyme system from the sulfur cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- An ArsR-type transcriptional regulator, SoxR, regulates sulfur oxidization (Sox) system in Cupriavidus pinatubonensis JMP134.BMC microbiology · 2026Article
- The structure of Mycobacterium thermoresistibile MmpS5 reveals a conserved disulfide bond across mycobacteria.Metallomics : integrated biometal science · 2024Article
- In the AlphaproteobacteriumAntioxidants (Basel, Switzerland) · 2023Article
- Generation and Physiology of Hydrogen Sulfide and Reactive Sulfur Species in Bacteria.Antioxidants (Basel, Switzerland) · 2022Review
- Conformational Selection Governs Carrier Domain Positioning inBiochemistry · 2022Article
- A deep-sea sulfate-reducing bacterium generates zero-valent sulfur via metabolizing thiosulfate.mLife · 2022Article
- Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data.Frontiers in microbiology · 2022Article
- The Heterotrophic Bacterium Cupriavidus pinatubonensis JMP134 Oxidizes Sulfide to Sulfate with Thiosulfate as a Key Intermediate.Applied and environmental microbiology · 2020Article
- Out of the Abyss: Genome and Metagenome Mining Reveals Unexpected Environmental Distribution of Abyssomicins.Frontiers in microbiology · 2020Article
- Methodologies for "Wiring" Redox Proteins/Enzymes to Electrode Surfaces.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Review
- Sulfur Oxidation in the Acidophilic AutotrophicFrontiers in microbiology · 2018Review
- Intermediates in the Sox sulfur oxidation pathway are bound to a sulfane conjugate of the carrier protein SoxYZ.PloS one · 2017Article
- Sulfur Metabolism ofFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The bacterial Sox (sulfur oxidation) pathway is an important route for the oxidation of inorganic sulfur compounds. Intermediates in the Sox pathway are covalently attached to the heterodimeric carrier protein SoxYZ through conjugation to a cysteine on a protein swinging arm. We have investigated how the carrier protein shuttles intermediates between the enzymes of the Sox pathway using the interaction between SoxYZ and the enzyme SoxB as our model. The carrier protein and enzyme interact only weakly, but we have trapped their complex by using a "suicide enzyme" strategy in which an engineered cysteine in the SoxB active site forms a disulfide bond with the incoming carrier arm cysteine. The structure of this trapped complex, together with calorimetric data, identifies sites of protein-protein interaction both at the entrance to the enzyme active site tunnel and at a second, distal, site. We find that the enzyme distinguishes between the substrate and product forms of the carrier protein through differences in their interaction kinetics and deduce that this behavior arises from substrate-specific stabilization of a conformational change in the enzyme active site. Our analysis also suggests how the carrier arm-bound substrate group is able to outcompete the adjacent C-terminal carboxylate of the carrier arm for binding to the active site metal ions. We infer that similar principles underlie carrier protein interactions with other enzymes of the Sox pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.