Evidence map›Paper›PMID 26653760›Full record

ArticleEndocrinology2016

Intermittent Fasting Promotes Fat Loss With Lean Mass Retention, Increased Hypothalamic Norepinephrine Content, and Increased Neuropeptide Y Gene Expression in Diet-Induced Obese Male Mice.

Juliet D Gotthardt, Jessica L Verpeut, Bryn L Yeomans, Jennifer A Yang, Ali Yasrebi, Troy A Roepke, Nicholas T Bello

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04259879 (Evaluation of p21 Induction and Molecular Pathways Related to Short-term Fasting Response), which is not on this map. Cited by 58 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed, 4 pooled it
5.3field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04259879 nacompletednot on this map

Evaluation of p21 Induction and Molecular Pathways Related to Short-term Fasting Response

TypeinterventionalSponsorIMDEA FoodRan2016 to 2016Enrolled20ConditionsFastingArmsFasting
3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 4 syntheses or guidelines pooled it, 121 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Pooled it
  5. Trial
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Metabolic gene signature in white adipose tissue of oral doses raspberry ketone [4-(4-hydroxyphenyl)-2-butanone] that prevent diet-induced weight gain and induce loss of righting reflex.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2023
    Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Juliet D GotthardtDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Jessica L VerpeutDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Bryn L YeomansDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Jennifer A YangDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Ali YasrebiDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Troy A RoepkeDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Nicholas T BelloDepartment of Animal Sciences (J.D.G., J.L.V., B.L.Y., J.A.Y., A.Y., T.A.R., N.T.B.), School of Environmental and Biological Sciences, Nutritional Sciences Graduate Program (J.D.G., B.L.Y., T.A.R., N.T.B.), Graduate Program in Endocrinology and Animal Biosciences (J.L.V., J.A.Y., T.A.R., N.T.B.), and New Jersey Institute for Food, Nutrition, and Health (T.A.R., N.T.B.), Rutgers, The State University of New Jersey, New Brunswick, New Jersey 0890.
Rutgers, The State University of New Jersey · US

Funding

Translational Research Support CoreP30ES005022 · NIEHS · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI ROBERT John LAUMBACH · 1988 to 2026
$47.4M
Estrogen Regulation of Channels Involved in the Control of Energy HomeostatisR00DK083457 · NIDDK · RUTGERS, THE STATE UNIV OF N.J. · PI ROEPKE, TROY ADAM · 2011 to 2014
$925k
NIDDK NIH HHS R00 DK083457NIDDK NIH HHS R00DK083457NIDDK NIH HHS R00DK83457-S1NIEHS NIH HHS P30 ES005022NIEHS NIH HHS P30ES005022
6 · The paper itself

Abstract

Clinical studies indicate alternate-day, intermittent fasting (IMF) protocols result in meaningful weight loss in obese individuals. To further understand the mechanisms sustaining weight loss by IMF, we investigated the metabolic and neural alterations of IMF in obese mice. Male C57/BL6 mice were fed a high-fat diet (HFD; 45% fat) ad libitum for 8 weeks to promote an obese phenotype. Mice were divided into four groups and either maintained on ad libitum HFD, received alternate-day access to HFD (IMF-HFD), and switched to ad libitum low-fat diet (LFD; 10% fat) or received IMF of LFD (IMF-LFD). After 4 weeks, IMF-HFD (∼13%) and IMF-LFD (∼18%) had significantly lower body weights than the HFD. Body fat was also lower (∼40%-52%) in all diet interventions. Lean mass was increased in the IMF-LFD (∼12%-13%) compared with the HFD and IMF-HFD groups. Oral glucose tolerance area under the curve was lower in the IMF-HFD (∼50%), whereas the insulin tolerance area under the curve was reduced in all diet interventions (∼22%-42%). HPLC measurements of hypothalamic tissue homogenates indicated higher (∼55%-60%) norepinephrine (NE) content in the anterior regions of the medial hypothalamus of IMF compared with the ad libitum-fed groups, whereas NE content was higher (∼19%-32%) in posterior regions in the IMF-LFD group only. Relative gene expression of Npy in the arcuate nucleus was increased (∼65%-75%) in IMF groups. Our novel findings indicate that intermittent fasting produces alterations in hypothalamic NE and neuropeptide Y, suggesting the counterregulatory processes of short-term weight loss are associated with an IMF dietary strategy.

Indexed as

Adipose TissueAnimalsAnterior Hypothalamic NucleusArcuate Nucleus of HypothalamusBody WeightChromatography, High Pressure LiquidDiet, High-FatFastingGene ExpressionGlucose Tolerance TestHypothalamusMaleMiceMice, Inbred C57BLNeuropeptide YNorepinephrineNeuropeptide YNorepinephrineRNA, Messenger

Identifiers

PMID26653760
PMCPMC4733124
OpenAlexW2220004601

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.