ArticleStem cell reports2015
Canonical MicroRNA Activity Facilitates but May Be Dispensable for Transcription Factor-Mediated Reprogramming.
Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Regulation and dysregulation of microRNA - transcription factor axes in differentiation and neuroblastoma.Cellular and molecular life sciences : CMLS · 2025Review
- The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.Stem cells (Dayton, Ohio) · 2025Article
- The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.bioRxiv : the preprint server for biology · 2024Article
- MicroRNA‑124: an emerging therapeutic target in central nervous system disorders.Experimental brain research · 2023Review
- Generation of inducible pluripotent stem cell lines from Alzheimer's disease patients with APOE e3/e3 genotype.Stem cell research · 2021Article
- Reprogramming of Pluripotency-Specific microRNA Signatures Is Not Essential to Generate Inducible Pluripotent Stem Cells.Cellular reprogramming · 2020Article
- microRNA-Based Biomarkers in Alzheimer's Disease (AD).Frontiers in neuroscience · 2020Review
- Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis.Nature communications · 2019Article
- Noncoding RNAs in the Regulation of Pluripotency and Reprogramming.Stem cell reviews and reports · 2018Review
- Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem Cells.Stem cell reports · 2017Article
- A DGCR8-Independent Stable MicroRNA Expression Strategy Reveals Important Functions of miR-290 and miR-183-182 Families in Mouse Embryonic Stem Cells.Stem cell reports · 2017Article
- Small regulators making big impacts: regulation of neural stem cells by small non-coding RNAs.Neural regeneration research · 2017Article
- Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem Cells.Stem cells and development · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are important regulators of reprogramming of somatic cells into induced pluripotent stem cells (iPSCs); however, it is unclear whether miRNAs are required for reprogramming and whether miRNA activity as a whole facilitates reprogramming. Here we report on successful reprogramming of mouse fibroblasts and neural stem cells (NSCs) lacking Dgcr8, a factor required for the biogenesis of canonical miRNAs, by Yamanaka factors, albeit at decreased efficiencies. Though iPSCs derived from Dgcr8-deficient mouse fibroblasts or NSCs were able to self-renew and expressed pluripotency-associated markers, they exhibited poor differentiation potential into mature somatic tissues, similar to Dgcr8(-/-) embryonic stem cells. The differentiation defects could be rescued with expression of DGCR8 cDNA. Our data demonstrate that while miRNA activity as a whole facilitates reprogramming, canonical miRNA may be dispensable in the derivation of iPSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.