ArticlePlant cell reports2016
Cytokinin response factor 4 (CRF4) is induced by cold and involved in freezing tolerance.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 106 citations in OpenAlex.
- Metabolomic and Transcriptomic Responses ofPlants (Basel, Switzerland) · 2026Article
- A single-cell screening platform accelerates functional genetics in plants.Nature biotechnology · 2026Article
- Phytohormone-Mediated Regulation of Plant Cold Stress Tolerance: Signaling, Hormonal Crosstalk, and Translational Perspectives.International journal of molecular sciences · 2026Review
- Genomic Insights into Winter Wheat Breeding for Severely Cold Climates.International journal of molecular sciences · 2026Article
- Moderate nitrogen supply alleviates low temperature stress by regulating nitrogen assimilation and hormone signaling in wheat tillering nodes.BMC plant biology · 2025Article
- Pangenome-Wide Identification, Evolutionary Analysis of Maize ZmPLD Gene Family, and Functional Validation of ZmPLD15 in Cold Stress Tolerance.Plants (Basel, Switzerland) · 2025Article
- Transcriptional signatures associated with waterlogging stress responses and aerenchyma formation in barley root tissue.Annals of botany · 2025Article
- Dynamic transcriptomic features accompanying the onset of first flowering in roses.BMC plant biology · 2025Article
- Genome-Wide Identification of Cytokinin Response Factors (CRFs) Involved in Stress Responses in Banana (International journal of molecular sciences · 2025Article
- Analysis of the AP2/ERF transcription factor family in Eriobotrya japonica and its role in exogenous melatonin-mediated regulation of salt stress.Functional & integrative genomics · 2025Article
- Molecular Networks Governing Plant Responses to Heat and Cold Stress.Plants (Basel, Switzerland) · 2025Review
- Non-Canonical Inter-Protein Interactions of Key Proteins Belonging to Cytokinin Signaling Pathways.Plants (Basel, Switzerland) · 2025Review
- Review
- Genome-wide identification of CRF gene family members in four rice subspecies and expression analysis of OsCRF members in response to cold stress at seedling stage.Scientific reports · 2024Article
- Functional Characterization of Accessible Chromatin in Common Wheat.International journal of molecular sciences · 2024Article
- Genome-Wide Investigation of the CRF Gene Family in Maize and Functional Analysis of ZmCRF9 in Response to Multiple Abiotic Stresses.International journal of molecular sciences · 2024Article
- Understanding AP2/ERF Transcription Factor Responses and Tolerance to Various Abiotic Stresses in Plants: A Comprehensive Review.International journal of molecular sciences · 2024Review
- CRF transcription factors in the trade-off between abiotic stress response and plant developmental processes.Frontiers in genetics · 2024Review
- Article
- Metabolic and Molecular Rearrangements of Sauvignon Blanc (Plants (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageCytokinin response factor 4 (CRF4) shows a short-term induction by cold (4 °C) that appears to play a role in non-acclimated freezing tolerance as seen in mutant and overexpression lines. Responses to abiotic stresses, such as cold stress, are critical to plant growth and optimal production. Examination of Arabidopsis cytokinin response factors (CRFs) showed transcriptional induction after exposure to cold (4 °C). In particular, CRF4 was strongly induced in both root and shoot tissues. As CRF4 is one of several CRFs not transcriptionally regulated by cytokinin, we further investigated its response to cold. Peak CRF4 induction occurred 6 h post cold exposure, after which expression was maintained at moderately elevated levels during extended cold and subsequent treatment recovery. Examination of CRF4 mutant and overexpression lines under standard (non-cold) conditions revealed little difference from WT. One exception was a small, but significant increase in primary root growth of overexpression plants (CRF4OX). Under cold conditions, the only phenotype observed was a reduction in the rate of germination of CRF4OX seeds. The pattern of CRF4 expression along with the lack of strong phenotype at 4 °C led us to hypothesize that cold induction of CRF4 could play a role in short-term cold acclimation leading to increased freeze tolerance. Examination of CRF4OX and crf4 plants exposed to freezing temperatures revealed mutants lacking expression of CRF4 were more sensitive to freezing, while CRF4OXs with increased levels CRF4 levels were more tolerant. Altered transcript expression of CBF and COR15a cold signaling pathway genes in crf4 mutant and overexpression lines suggest that CRF4 may be potentially connected to this pathway. Overall this indicates that CRF4 plays an important role in both cold response and freezing stress.
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